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General |
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Antigen | Interferon Regulatory Factor 5 (IRF5) Antikörper |
Epitop | C-Term Alternativen |
Reaktivität | Rind (Kuh), Human, Maus, Ratte (Rattus) Alternativen |
Wirt | Ziege Alternativen |
Klonalität | |
Konjugat | Dieser IRF5 Antikörper ist unkonjugiert Alternativen |
Applikation |
Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternativen
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Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-IRF5 AntikörperTarget Details IRF5 Anwendungsinformationen Handhabung Bilder |
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Sequenz | C-QGPWPMHPAG MQ |
Spezifität | This antibody is expected to recognize all four reported isoforms. |
Kreuzreaktivität (Details) |
Species reactivity (expected):Mouse, Rat, Cow. Species reactivity (tested):Human. |
Reinigung | Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide |
Immunogen | Peptide from C Terminus of the protein sequence according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1. Genename: IRF5 |
Plasmids, Primers & others | |
Target Details IRF5Produktdetails anti-IRF5 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | IRF5 (IRF5 Antibody Abstract) |
Hintergrund | Interferon regulatory factors (IRFs) are transcription factors that possess a novel helix turn helix DNA binding motif and mediate virus and interferon (IFN) induced signaling pathways. The IRF5 protein is structurally related to IRF6. Like other IRFs, IRF5 and IRF6 contain 5 conserved tryptophan residues in the 115 amino acid N terminal DNA binding domain. IRF5 has a central IRF association domain (IAD) and, like IRF7 and IRF8, is induced by IFNA/IFNB stimulation. IRF5 has a type I IFN transactivation potential similar to that of IRF1. IRF3 and IRF5 form heterodimers and are both required for effective induction of IFNA gene transcription.Synonyms: IRF-5, Interferon regulatory factor 5 |
Gen-ID | 3663 |
NCBI Accession | NP_002191 |
UniProt | Q13568 |
Pathways | TLR Signalweg, Autophagie |
AnwendungsinformationenProduktdetails anti-IRF5 Antikörper Target Details IRF5 Handhabung Bilder zurück nach oben |
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Applikations-hinweise |
Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 57 kDa band observed in lysates of cell line A549. Intransfected HEK293 transiently expressing IRF5 a strong band of approx.67 kDa isobserved. This band is not observed in the non-transfected HEK293. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-IRF5 Antikörper Target Details IRF5 Anwendungsinformationen Bilder zurück nach oben |
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Konzentration | 0.5 mg/mL |
Buffer | Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handhabung | Avoid repeated freezing and thawing. |
Lagerung | 4 °C/-20 °C |
Informationen zur Lagerung | Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C. |