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21.863953 | ABIN2783229 | IHC WB | Rabbit | N-Term | Anmelden zum Anzeigen | Polyclonal | 1 | |||
18.863953 | ABIN2783230 | IHC WB | Rabbit | N-Term | Anmelden zum Anzeigen | Polyclonal | 1 | |||
7 | ABIN321373 | IHC IHC (p) WB | Rabbit | IgG | N-Term | Anmelden zum Anzeigen | Polyclonal | 0 |
General |
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Antigen | CD36 Molecule (thrombospondin Receptor) (CD36) Antikörper |
Epitop | AA 100-200 Alternativen |
Reaktivität | Rind (Kuh), Human, Maus, Schwein, Primate, Ratte (Rattus) Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Dieser CD36 Antikörper ist unkonjugiert Alternativen |
Applikation |
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
Alternativen
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47 Publikationen vorhanden |
Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-CD36 AntikörperTarget Details CD36 Anwendungsinformationen Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder |
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Reinigung | Immunogen affinity purified |
Immunogen | A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671] |
Plasmids, Primers & others | |
Target Details CD36Produktdetails anti-CD36 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | CD36 (CD36 Antibody Abstract) |
Hintergrund | Gene Symbol: CD36 |
Molekulargewicht | Theoretical MW: 75 kDa |
Gen-ID | 948 |
UniProt | P16671 |
Pathways | TLR Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism |
AnwendungsinformationenProduktdetails anti-CD36 Antikörper Target Details CD36 Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder zurück nach oben |
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Applikations-hinweise | Western Blot 1:500-1:2000, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-FrozenThis CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Kommentare |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Protokoll |
Protocol specific for CD36 Antibody Western Blot Protocol 1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50ug of total protein per lane. . Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus. . Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil. . Rinse the blot in TBST for approximately 5 minutes. . Block the membrane using 5 % non-fat dry milk, 1 % BSA in TBST for 1 hour. . Dilute the rabbit anti-CD36 primary antibody in blocking buffer and incubate overnight at 4 degrees Celcius. . Wash the membrane in TBST 5 times for 5 minutes each. . Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature. . Wash the blot in TBST 5 times for 5 minutes each. . Apply the detection reagent of choice in accordance with the manufacturers instructions.IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-CD36 Antikörper Target Details CD36 Anwendungsinformationen Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder zurück nach oben |
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Format | Liquid |
Konzentration | 1 mg/mL |
Buffer |
PBS Buffer contains: 0.02 % Sodium Azide |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handhabung | Do not freeze. |
Lagerung | 4 °C,-20 °C |
Informationen zur Lagerung | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Referenzen für anti-CD36 Antikörper (ABIN152911)Produktdetails anti-CD36 Antikörper Target Details CD36 Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Produkt verwendet in: |
Lee, Han, Park, Baek, Song, Choi, Takuwa, Ryu, Kim, He, Schuchman, Bae, Jin: "Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease." in: Nature communications, Vol. 9, Issue 1, pp. 1479, 2018 Rozovski, Harris, Li, Liu, Jain, Ferrajoli, Burger, Thompson, Jain, Wierda, Keating, Estrov: "STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells." in: Oncotarget, Vol. 9, Issue 30, pp. 21268-21280, 2018 Misra, Feng, Chandran, Kabir, Rotllan, Aryal, Sheikh, Ding, Qin, Fernández-Hernando, Tellides, Greif: "Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells." in: Nature communications, Vol. 9, Issue 1, pp. 2073, 2018 Sankar, Donegan, Shah, Reddi, Wood: "Heme and hemoglobin suppress amyloid β-mediated inflammatory activation of mouse astrocytes." in: The Journal of biological chemistry, Vol. 293, Issue 29, pp. 11358-11373, 2018 Li, Lu, Ru, Lopes-Virella, Lyons, Huang: "Saturated Fatty Acid Combined with Lipopolysaccharide Stimulates a Strong Inflammatory Response in Hepatocytes in vivo and in vitro." in: American journal of physiology. Endocrinology and metabolism, 2018 Fang, Wang, Yao, Li, Wang, Liu, Tao, Sun, Liao: "The adhesion and migration of microglia to β-amyloid (Aβ) is decreased with aging and inhibited by Nogo/NgR pathway." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 210, 2018 Lavie, Dyugovskaya, Polyakov, Rogovoy, Leder: "Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro." in: Journal of visualized experiments : JoVE, Issue 119, 2017 Chen, Huang, Yang, Xin, Cui, Xie, Silverstein: "Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 8, pp. 1462-1469, 2017 (Probematerial (Species): Mouse (Murine)). Xian, Ding, Dieckmann, Zhou, Plattner, Liu, Parks, Hammer, Boucher, Tsai, Herz: "LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis." in: eLife, Vol. 6, 2017 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)). Guri, Colombi, Dazert, Hindupur, Roszik, Moes, Jenoe, Heim, Riezman, Riezman, Hall: "mTORC2 Promotes Tumorigenesis via Lipid Synthesis." in: Cancer cell, Vol. 32, Issue 6, pp. 807-823.e12, 2017 (Probematerial (Species): Mouse (Murine)). Souza, Bocharov, Baranova, Vishnyakova, Huang, Wilkins, Hu, Street, Alvarez-Prats, Mullick, Patterson, Remaley, Eggerman, Yuen, Star: "Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation." in: Kidney international, Vol. 89, Issue 4, pp. 809-22, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections) Salameh, Daquinag, Staquicini, An, Hajjar, Pasqualini, Arap, Kolonin: "Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue." in: JCI insight, Vol. 1, Issue 10, 2016 Garbacz, Lu, Miller, Poloyac, Eyre, Mayrhofer, Xu, Ren, Xie: "Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance." in: Molecular and cellular biology, Vol. 36, Issue 21, pp. 2715-2727, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting Sachdev, Leopold, Bauer, Patankar, Iqbal, Obrowsky, Boverhof, Doktorova, Scheicher, Goeritzer, Kolb, Turnbull, Zimmer, Hoefler, Hussain, Groen, Kratky: "Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism." in: Biochimica et biophysica acta, Vol. 1861, Issue 9 Pt A, pp. 1132-41, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) Vaillant, Lauzier, Ruiz, Shi, Lachance, Rivard, Bolduc, Thorin, Tardif, Des Rosiers: "Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 311, Issue 4, pp. H991-H1003, 2016 Konstantynowicz-Nowicka, Harasim, Baranowski, Chabowski: "New evidence for the role of ceramide in the development of hepatic insulin resistance." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116858, 2015 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting Zidek, Ackermann, Hartleben, Eichwald, Kortman, Kiehntopf, Leutz, Sonenberg, Wang, von Maltzahn, Müller, Calkhoven: "Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice." in: EMBO reports, Vol. 16, Issue 8, pp. 1022-36, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting Barroso, Rodríguez-Rodríguez, Chacón, Maymó-Masip, Ferrer, Salvadó, Salmerón, Wabistch, Palomer, Vendrell, Wahli, Vázquez-Carrera: "PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation." in: Biochimica et biophysica acta, Vol. 1852, Issue 5, pp. 1049-58, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting Bartels, Christoffersen, Lindholm, Nielsen: "Altered metabolism of LDL in the arterial wall precedes atherosclerosis regression." in: Circulation research, Vol. 117, Issue 11, pp. 933-42, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry Zhao, Xiong, Lechner, Klenotic, Hamburg, Hulver, Khare, Oriss, Mangalmurti, Chan, Zhang, Ross, Stolz, Rosengart, Pilewski, Ray, Ray, Silverstein, Lee: "Thrombospondin-1 triggers macrophage IL-10 production and promotes resolution of experimental lung injury." in: Mucosal immunology, Vol. 7, Issue 2, pp. 440-8, 2014 |
BilderProduktdetails anti-CD36 Antikörper Target Details CD36 Anwendungsinformationen Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) zurück nach oben |
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Bilder des Herstellers |
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