anti-Maus CD36 Antikörper für Immunohistochemistry

Recommended CD36 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD36 Molecule (thrombospondin Receptor) (CD36) Antikörper
  • BDPLT10
  • CHDS7
  • FAT
  • GP3B
  • GP4
  • GPIV
  • PASIV
  • SCARB3
  • Fat
  • Scarb3
  • GPIIIB
  • PAS-4
  • zgc:92513
  • CD36 molecule
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Cd36
  • cd36
Epitop
AA 100-200
20
16
15
11
6
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Human, Maus, Schwein, Primate, Ratte (Rattus)
359
153
90
8
7
4
3
3
3
3
1
1
1
Wirt
Kaninchen
248
138
52
12
2
Klonalität
Polyklonal
Konjugat
Dieser CD36 Antikörper ist unkonjugiert
53
28
23
20
10
9
8
5
5
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
291
142
127
91
65
41
40
39
35
24
14
3
3
3
3
3
3
2
2
2
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN152911
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
9.312915 ABIN2783229 IHC WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 1
9.312915 ABIN258619 IHC (p) IP IHC WB Mouse IgA kappa Anmelden zum Anzeigen D-2712 7
9.312915 ABIN4900173 CyTOF FACS IHC Rat IgG1 AA 30-439 Anmelden zum Anzeigen 324205 2
9.312915 ABIN2469712 IHC ELISA WB Rabbit IgG AA 99 - 114 Anmelden zum Anzeigen Polyclonal 0
9.312915 ABIN2432795 IHC ELISA Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN2650903 FACS IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1732841 IHC WB Rabbit AA 100-200 Anmelden zum Anzeigen Polyclonal 0
1 ABIN269593 Func IA ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Anmelden zum Anzeigen FA6-152 4
1 ABIN2657411 FACS IF IP IHC Alexa Fluor 488 Hamster IgG Anmelden zum Anzeigen HM36 5
1 ABIN2618488 IHC IHC (p) WB Rabbit IgG AA 99-114 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2561720 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN2739295 IHC IHC (p) WB Rabbit IgG AA 31-66 Anmelden zum Anzeigen Polyclonal 0
1 ABIN377519 IHC WB Rabbit IgG Extracellular Domain Anmelden zum Anzeigen Polyclonal 0
1 ABIN2657766 FACS IF IHC Alexa Fluor 647 Hamster IgG Anmelden zum Anzeigen HM36 3
1 ABIN2661064 FACS IF IHC Biotin Hamster IgG Anmelden zum Anzeigen HM36 3
1 ABIN1582257 IHC ELISA WB Rabbit N-Term Anmelden zum Anzeigen Polyclonal 1
1 ABIN409707 IHC IHC (p) WB Biotin Mouse IgA kappa Anmelden zum Anzeigen D-2712 0
1 ABIN409709 IHC IHC (p) WB DyLight 488 Mouse IgA kappa Anmelden zum Anzeigen D-2712 0
1 ABIN2887447 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN409710 IHC IHC (p) WB HRP Mouse IgA kappa Anmelden zum Anzeigen D-2712 0

General

Antigen CD36 Molecule (thrombospondin Receptor) (CD36) Antikörper
Epitop AA 100-200
(20), (16), (15), (11), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Rind (Kuh), Human, Maus, Schwein, Primate, Ratte (Rattus)
(359), (153), (90), (8), (7), (4), (3), (3), (3), (3), (1), (1), (1)
Wirt Kaninchen
(248), (138), (52), (12), (2)
Klonalität
Polyklonal
Konjugat Dieser CD36 Antikörper ist unkonjugiert
(53), (28), (23), (20), (10), (9), (8), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(291), (142), (127), (91), (65), (41), (40), (39), (35), (24), (14), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 41 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD36 Antikörper

Target Details CD36 Anwendungsinformationen Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder
Reinigung Immunogen affinity purified
Immunogen A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]
Plasmids, Primers & others

Target Details CD36

Produktdetails anti-CD36 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD36 (CD36 Antibody Abstract)
Hintergrund Gene Symbol: CD36
Molekulargewicht Theoretical MW: 75 kDa
Gen-ID 948
UniProt P16671
Pathways TLR Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism

Anwendungsinformationen

Produktdetails anti-CD36 Antikörper Target Details CD36 Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder zurück nach oben
Applikations-hinweise Western Blot 1:500-1:2000, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-FrozenThis CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for CD36 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBST for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk, 1 % BSA in TBST for 1 hour.
. Dilute the rabbit anti-CD36 primary antibody in blocking buffer and incubate overnight at 4 degrees Celcius.
. Wash the membrane in TBST 5 times for 5 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBST 5 times for 5 minutes each.
. Apply the detection reagent of choice in accordance with the manufacturers instructions.IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD36 Antikörper Target Details CD36 Anwendungsinformationen Referenzen für anti-CD36 Antikörper (ABIN152911) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-CD36 Antikörper (ABIN152911)

Produktdetails anti-CD36 Antikörper Target Details CD36 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Lavie, Dyugovskaya, Polyakov, Rogovoy, Leder: "Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro." in: Journal of visualized experiments : JoVE, Issue 119, 2017

Chen, Huang, Yang, Xin, Cui, Xie, Silverstein: "Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 8, pp. 1462-1469, 2017 (Probematerial (Species): Mouse (Murine)).

Guri, Colombi, Dazert, Hindupur, Roszik, Moes, Jenoe, Heim, Riezman, Riezman, Hall: "mTORC2 Promotes Tumorigenesis via Lipid Synthesis." in: Cancer cell, Vol. 32, Issue 6, pp. 807-823.e12, 2017 (Probematerial (Species): Mouse (Murine)).

Souza, Bocharov, Baranova, Vishnyakova, Huang, Wilkins, Hu, Street, Alvarez-Prats, Mullick, Patterson, Remaley, Eggerman, Yuen, Star: "Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation." in: Kidney international, Vol. 89, Issue 4, pp. 809-22, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Salameh, Daquinag, Staquicini, An, Hajjar, Pasqualini, Arap, Kolonin: "Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue." in: JCI insight, Vol. 1, Issue 10, 2016

Garbacz, Lu, Miller, Poloyac, Eyre, Mayrhofer, Xu, Ren, Xie: "Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance." in: Molecular and cellular biology, Vol. 36, Issue 21, pp. 2715-2727, 2016 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Sachdev, Leopold, Bauer, Patankar, Iqbal, Obrowsky, Boverhof, Doktorova, Scheicher, Goeritzer, Kolb, Turnbull, Zimmer, Hoefler, Hussain, Groen, Kratky: "Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism." in: Biochimica et biophysica acta, Vol. 1861, Issue 9 Pt A, pp. 1132-41, 2016 Von den Autoren verwendete Methode: Western Blotting (WB)

Vaillant, Lauzier, Ruiz, Shi, Lachance, Rivard, Bolduc, Thorin, Tardif, Des Rosiers: "Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 311, Issue 4, pp. H991-H1003, 2016

Konstantynowicz-Nowicka, Harasim, Baranowski, Chabowski: "New evidence for the role of ceramide in the development of hepatic insulin resistance." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116858, 2015 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Zidek, Ackermann, Hartleben, Eichwald, Kortman, Kiehntopf, Leutz, Sonenberg, Wang, von Maltzahn, Müller, Calkhoven: "Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice." in: EMBO reports, Vol. 16, Issue 8, pp. 1022-36, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Barroso, Rodríguez-Rodríguez, Chacón, Maymó-Masip, Ferrer, Salvadó, Salmerón, Wabistch, Palomer, Vendrell, Wahli, Vázquez-Carrera: "PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation." in: Biochimica et biophysica acta, Vol. 1852, Issue 5, pp. 1049-58, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Bartels, Christoffersen, Lindholm, Nielsen: "Altered metabolism of LDL in the arterial wall precedes atherosclerosis regression." in: Circulation research, Vol. 117, Issue 11, pp. 933-42, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Zhao, Xiong, Lechner, Klenotic, Hamburg, Hulver, Khare, Oriss, Mangalmurti, Chan, Zhang, Ross, Stolz, Rosengart, Pilewski, Ray, Ray, Silverstein, Lee: "Thrombospondin-1 triggers macrophage IL-10 production and promotes resolution of experimental lung injury." in: Mucosal immunology, Vol. 7, Issue 2, pp. 440-8, 2014

Khan, Abu Jawdeh, Goel, Schilling, Parker, Puchowicz, Yadav, Harris, El-Meanawy, Hoshi, Shinlapawittayatorn, Deschênes, Ficker, Schelling: "Lipotoxic disruption of NHE1 interaction with PI(4,5)P2 expedites proximal tubule apoptosis." in: The Journal of clinical investigation, Vol. 124, Issue 3, pp. 1057-68, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Frozen Sections)

Hale, Otvos, Sinyuk, Alvarado, Hitomi, Stoltz, Wu, Flavahan, Levison, Johansen, Schmitt, Neltner, Huang, Ren, Sloan, Silverstein, Gladson, DiDonato, Brown, McIntyre, Hazen, Horbinski, Rich, Lathia: "Cancer stem cell-specific scavenger receptor CD36 drives glioblastoma progression." in: Stem cells (Dayton, Ohio), Vol. 32, Issue 7, pp. 1746-58, 2014 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Chen, Yong, Xia, Wang, Liang, Zhang, Lu: "The excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36." in: Carbohydrate polymers, Vol. 112, pp. 16-23, 2014

Srikanthan, Li, Silverstein, McIntyre: "Exosome poly-ubiquitin inhibits platelet activation, downregulates CD36 and inhibits pro-atherothombotic cellular functions." in: Journal of thrombosis and haemostasis : JTH, Vol. 12, Issue 11, pp. 1906-17, 2014

Ye, Holland, Gordillo, Wang, Wang, Shao, Morley, Gupta, Stahl, Scherer: "Adiponectin is essential for lipid homeostasis and survival under insulin deficiency and promotes β-cell regeneration." in: eLife, Vol. 3, 2014 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry

Pienimaeki-Roemer, Ruebsaamen, Boettcher, Orsó, Scherer, Liebisch, Kilalic, Ahrens, Schmitz: "Stored platelets alter glycerophospholipid and sphingolipid species, which are differentially transferred to newly released extracellular vesicles." in: Transfusion, Vol. 53, Issue 3, pp. 612-26, 2013 (Probematerial (Species): Human). Weitere Details: Western Blotting

Olson, Ledee, Iwamoto, Kajimoto, OKelly Priddy, Isern, Portman: "C-Myc induced compensated cardiac hypertrophy increases free fatty acid utilization for the citric acid cycle." in: Journal of molecular and cellular cardiology, Vol. 55, pp. 156-64, 2013 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Bilder

Produktdetails anti-CD36 Antikörper Target Details CD36 Anwendungsinformationen Handhabung Referenzen für anti-CD36 Antikörper (ABIN152911) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml ABIN152911, ...
Immunohistochemistry (IHC) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Staining of humnan adipocytes using ABIN152911.
Immunofluorescence (IF) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody [ABIN152911] - CD36 antib...
Immunofluorescence (IF) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody - Mouse liver, image is ...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Total protein from Human Skin and Adipose tissue...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Alcohol feeding significantly up-regulated CD36 ...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Mouse primary muscle cell lysate. This image was...