anti-Non-Human Primate CD14 Antikörper für Functional Studies

Recommended CD14 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD14 Molecule (CD14) Antikörper
  • CD14 molecule
  • CD14 antigen
  • CD14
  • Cd14
Reaktivität
Human, Non-Human Primate
1048
163
117
104
100
99
81
73
72
65
59
52
42
40
36
27
17
17
15
12
9
9
7
3
2
1
1
1
Wirt
Maus
982
141
70
24
3
Klonalität
Monoklonal
Konjugat
Dieser CD14 Antikörper ist konjugiert mit PE
109
105
80
73
36
27
27
23
20
20
15
15
15
15
15
10
10
10
9
8
8
8
7
7
7
6
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
970
344
288
241
222
146
83
69
60
51
26
17
14
13
12
8
6
4
3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN93967
$ 366.85
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN93966 ELISA FACS Func IP WB FITC Mouse IgG1 Anmelden zum Anzeigen MEM-18 21
1 ABIN93965 ELISA FACS Func IP WB Biotin Mouse IgG1 Anmelden zum Anzeigen MEM-18 21
1 ABIN93963 Func FACS IP ELISA WB Mouse IgG1 Anmelden zum Anzeigen MEM-18 21
1 ABIN638430 Func FACS IP ELISA WB Mouse IgG1 Anmelden zum Anzeigen MEM-18 13
1 ABIN192089 ELISA FACS Func IP WB APC Mouse IgG1 Anmelden zum Anzeigen MEM-18 14

General

Antigen CD14 Molecule (CD14) Antikörper
Reaktivität Human, Non-Human Primate
(1048), (163), (117), (104), (100), (99), (81), (73), (72), (65), (59), (52), (42), (40), (36), (27), (17), (17), (15), (12), (9), (9), (7), (3), (2), (1), (1), (1)
Wirt Maus
(982), (141), (70), (24), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD14 Antikörper ist konjugiert mit PE
(109), (105), (80), (73), (36), (27), (27), (23), (20), (20), (15), (15), (15), (15), (15), (10), (10), (10), (9), (8), (8), (8), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(970), (344), (288), (241), (222), (146), (83), (69), (60), (51), (26), (17), (14), (13), (12), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 21 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD14 Antikörper

Target Details CD14 Anwendungsinformationen Handhabung Referenzen für anti-CD14 Antikörper (ABIN93967) Bilder
Spezifität The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
HLDA III, WS Code M 253
HLDA IV, WS Code M 314
HLDA V, WS Code M MA087
HLDA VI, WS Code M MA95
Produktmerkmale The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Klon MEM-18
Isotyp IgG1
Plasmids, Primers & others

Target Details CD14

Produktdetails anti-CD14 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD14 Antikörper (ABIN93967) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD14 (CD14 Antibody Abstract)
Hintergrund CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-CD14 Antikörper Target Details CD14 Handhabung Referenzen für anti-CD14 Antikörper (ABIN93967) Bilder zurück nach oben
Applikations-hinweise The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD14 Antikörper Target Details CD14 Anwendungsinformationen Referenzen für anti-CD14 Antikörper (ABIN93967) Bilder zurück nach oben
Rekonstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Referenzen für anti-CD14 Antikörper (ABIN93967)

Produktdetails anti-CD14 Antikörper Target Details CD14 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Miko?ajczyk, Skrzeczy?ska-Moncznik, Zarebski, Marewicz, Wi?niewska, Dzieba, Dobrucki, Pryjma: "Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells." in: Immunology, Vol. 128, Issue 1, pp. 103-13, 2009

Kuronuma, Mitsuzawa, Takeda, Nishitani, Chan, Kuroki, Nakamura, Voelker: "Anionic pulmonary surfactant phospholipids inhibit inflammatory responses from alveolar macrophages and U937 cells by binding the lipopolysaccharide-interacting proteins CD14 and MD-2." in: The Journal of biological chemistry, Vol. 284, Issue 38, pp. 25488-500, 2009

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009

Jursik, Prchal, Grillari-Voglauer, Drbal, Fuertbauer, Jungfer, Albert, Steinhuber, Hemetsberger, Grillari, Stockinger, Katinger: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009

von Schlieffen, Oskolkova, Schabbauer, Gruber, Blueml, Genest, Kadl, Marsik, Knapp, Chow, Leitinger, Binder, Bochkov: "Multi-hit inhibition of circulating and cell-associated components of the toll-like receptor 4 pathway by oxidized phospholipids." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, Issue 3, pp. 356-62, 2009

Wu, Zhang, Zhang, Tang, Ren, Dai: "Expansion of CD14+CD16+ peripheral monocytes among patients with aseptic loosening." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 58, Issue 9, pp. 561-70, 2009

Schrauf, Kirchberger, Majdic, Seyerl, Zlabinger, Stuhlmeier, Sachet, Seipelt, Stöckl: "The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 7, pp. 4440-8, 2009

Schwartzkopff, Grimm, Lankford, Fields, Wang, Brandt, Clouse: "Platelet factor 4 (CXCL4) facilitates human macrophage infection with HIV-1 and potentiates virus replication." in: Innate immunity, 2009

Lødrup Carlsen, Granum: "Soluble CD14: role in atopic disease and recurrent infections, including otitis media." in: Current allergy and asthma reports, Vol. 7, Issue 6, pp. 436-43, 2007

Asai, Makimura, Kawabata, Ogawa: "Soluble CD14 discriminates slight structural differences between lipid as that lead to distinct host cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 11, pp. 7674-83, 2007

Fernández-Real, Broch, Richart, Vendrell, López-Bermejo, Ricart: "CD14 monocyte receptor, involved in the inflammatory cascade, and insulin sensitivity." in: The Journal of clinical endocrinology and metabolism, Vol. 88, Issue 4, pp. 1780-4, 2003

Juan, Hailman, Kelley, Wright, Lichenstein: "Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding." in: The Journal of biological chemistry, Vol. 270, Issue 29, pp. 17237-42, 1995

Produkt verwendet in:

Weiss, Lichtenauer, Kirchner, Stock, Aurich, Christ, Brockhoff, Kunz-Schughart, Jauch, Schlitt, Thasler: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007

Iwaki, Nishitani, Mitsuzawa, Hyakushima, Sano, Kuroki: "The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain." in: Biochemical and biophysical research communications, Vol. 328, Issue 1, pp. 173-6, 2005

Sing, Rost, Tvardovskaia, Roggenkamp, Wiedemann, Kirschning, Aepfelbacher, Heesemann: "Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1017-24, 2002

Stöckl, Majdic, Fischer, Maurer, Knapp: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001

Funda, Tucková, Farré, Iwase, Moro, Tlaskalová-Hogenová: "CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited." in: Infection and immunity, Vol. 69, Issue 6, pp. 3772-81, 2001

Bazil, Baudys, Hilgert, Stefanová, Low, Zbrozek, Horejsí: "Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14." in: Molecular immunology, Vol. 26, Issue 7, pp. 657-62, 1989

Bilder

Produktdetails anti-CD14 Antikörper Target Details CD14 Anwendungsinformationen Handhabung Referenzen für anti-CD14 Antikörper (ABIN93967) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-CD14 Molecule (CD14) antibody (PE) (ABIN93967) Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 usin...
Flow Cytometry (FACS) image for anti-CD14 Molecule (CD14) antibody (PE) (ABIN93967) Surface staining of human peripheral blood leukocytes using anti-human CD14