anti-Maus FEN1 Antikörper für Immunofluorescence

Recommended FEN1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Flap Structure-Specific Endonuclease 1 (FEN1) Antikörper
  • FEN-1
  • FEN1
  • AW538437
  • MF1
  • RAD2
  • cb879
  • fen-1
  • fen1
  • mf1
  • rad2
  • xFEN1a
  • flap structure-specific endonuclease 1
  • flap endonuclease 1-like
  • Flap endonuclease-1
  • 5'-3' exonuclease family protein
  • flap structure specific endonuclease 1
  • flap structure-specific endonuclease 1 L homeolog
  • nuclease, Rad2 family
  • FEN1
  • LOC100808044
  • Bm1_49605
  • AT5G26680
  • Fen1
  • fen1
  • fen1.L
Epitop
Center
16
8
7
7
6
6
4
4
3
3
3
2
2
2
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
144
84
56
7
7
6
6
6
4
4
4
3
1
1
1
1
Wirt
Kaninchen
93
51
Klonalität
Polyklonal
Konjugat
Dieser FEN1 Antikörper ist unkonjugiert
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
124
43
38
29
28
20
18
13
8
4
3
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2855166
$ 466.18
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
19.075058 ABIN3184606 ELISA IF IHC WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
15.525917 ABIN151083 ICC IF IP WB Mouse IgG1 Anmelden zum Anzeigen 4E7 17
14.58741 ABIN2706146 IC IF IP IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
14.58741 ABIN1533720 IF IHC ELISA WB Rabbit IgG AA 86-135 Anmelden zum Anzeigen Polyclonal 2
14.58741 ABIN6567251 IF IHC IP Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
14.58741 ABIN1576483 IF IHC ELISA WB Rabbit Anmelden zum Anzeigen Polyclonal 0
11.025917 ABIN4311324 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
10 ABIN2889469 ELISA IF IHC WB Rabbit IgG AA 86-135 Anmelden zum Anzeigen Polyclonal 0
10 ABIN6258158 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
7 ABIN272186 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN2892105 IF IHC IHC (p) WB Rabbit Ala119 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2620779 ICC IF IHC IHC (p) IP WB Rabbit Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN2888147 IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN5959480 ELISA IF IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
-11.561493 ABIN4311325 ICC IF WB DyLight 350 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
-11.561493 ABIN4311326 ICC IF WB DyLight 405 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
-11.561493 ABIN4271875 FACS ICC IF WB PerCP Mouse IgG1 Anmelden zum Anzeigen 4E7 0
-11.561493 ABIN4311329 ICC IF WB DyLight 488 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
-11.561493 ABIN4311331 ICC IF WB DyLight 680 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
-11.561493 ABIN4311328 ICC IF WB DyLight 755 Mouse IgG1 Anmelden zum Anzeigen 4E7 0

General

Antigen Flap Structure-Specific Endonuclease 1 (FEN1) Antikörper
Epitop Center
(16), (8), (7), (7), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(144), (84), (56), (7), (7), (6), (6), (6), (4), (4), (4), (3), (1), (1), (1), (1)
Wirt Kaninchen
(93), (51)
Klonalität
Polyklonal
Konjugat Dieser FEN1 Antikörper ist unkonjugiert
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(124), (43), (38), (29), (28), (20), (18), (13), (8), (4), (3), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FEN1 Antikörper

Target Details FEN1 Anwendungsinformationen Handhabung Bilder
Produktmerkmale Rabbit Polyclonal antibody to FEN1 (flap structure-specific endonuclease 1)
FEN1 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.
Isotyp IgG
Plasmids, Primers & others

Target Details FEN1

Produktdetails anti-FEN1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung FEN1 (FEN1 Antibody Abstract)
Hintergrund The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Cellular Localization: Nucleus
Molekulargewicht 43 kDa
Gen-ID 2237
Pathways Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA

Anwendungsinformationen

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Handhabung Bilder zurück nach oben
Applikations-hinweise Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Kommentare

Positive Control: HepG2 , NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.72 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungs-mittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A549, using FEN1, ...
Immunoprecipitation (IP) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) IP Image FEN1 antibody immunoprecipitates FEN1 protein in IP experiments. IP samples:...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 10% SDS PAGE antibody dilute...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) WB Image FEN1 antibody detects FEN1 protein by Western blot analysis. A. 30 µg PC-12 ...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) WB Image FEN1 antibody detects FEN1 protein by Western blot analysis. A. 30 µg NIH-3T...
Immunohistochemistry (IHC) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) IHC-P Image FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma b...