anti-Maus FEN1 Antikörper für Immunofluorescence

Recommended FEN1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Flap Structure-Specific Endonuclease 1 (FEN1) Antikörper
  • FEN-1
  • FEN1
  • AW538437
  • MF1
  • RAD2
  • cb879
  • fen-1
  • fen1
  • mf1
  • rad2
  • xFEN1a
  • flap structure-specific endonuclease 1
  • flap endonuclease 1-like
  • Flap endonuclease-1
  • 5'-3' exonuclease family protein
  • flap structure specific endonuclease 1
  • flap structure-specific endonuclease 1 L homeolog
  • nuclease, Rad2 family
  • FEN1
  • LOC100808044
  • Bm1_49605
  • AT5G26680
  • Fen1
  • fen1
  • fen1.L
Reaktivität
Human, Maus
141
80
53
7
7
6
6
6
4
4
4
3
1
1
1
1
Wirt
Maus
90
51
Klonalität
Monoklonal
Konjugat
Dieser FEN1 Antikörper ist unkonjugiert
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
122
44
37
29
25
20
19
13
8
6
3
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN151083
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.106882 ABIN2706146 IC IF IP IHC WB Rabbit Center Anmelden zum Anzeigen Polyclonal 0
12.106882 ABIN1533720 IF IHC ELISA WB Rabbit IgG AA 86-135 Anmelden zum Anzeigen Polyclonal 2
12.106882 ABIN2855166 ICC IF IP IHC (p) WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 0
12.106882 ABIN4311324 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
12.106882 ABIN5965680 IF IHC IP Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN2889469 ELISA IF IHC WB Rabbit IgG AA 86-135 Anmelden zum Anzeigen Polyclonal 0
1 ABIN272186 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN3184606 ELISA IF IHC WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN2892105 IF IHC IHC (p) WB Rabbit Ala119 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2888147 IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN2620779 ICC IF IHC IHC (p) IP WB Rabbit Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN4311333 ICC IF WB Alexa Fluor 488 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311335 ICC IF WB Alexa Fluor 700 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311329 ICC IF WB DyLight 488 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311332 ICC IF WB Alexa Fluor 405 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4271875 FACS ICC IF WB PerCP Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311325 ICC IF WB DyLight 350 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311328 ICC IF WB DyLight 755 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311331 ICC IF WB DyLight 680 Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN4311334 ICC IF WB Alexa Fluor 647 Mouse IgG1 Anmelden zum Anzeigen 4E7 0

General

Antigen Flap Structure-Specific Endonuclease 1 (FEN1) Antikörper
Reaktivität Human, Maus
(141), (80), (53), (7), (7), (6), (6), (6), (4), (4), (4), (3), (1), (1), (1), (1)
Wirt Maus
(90), (51)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser FEN1 Antikörper ist unkonjugiert
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(122), (44), (37), (29), (25), (20), (19), (13), (8), (6), (3), (1), (1)
Pubmed 17 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FEN1 Antikörper

Target Details FEN1 Anwendungsinformationen Handhabung Referenzen für anti-FEN1 Antikörper (ABIN151083) Bilder
Spezifität This is specific for FEN-1.
Reinigung Protein G purified
Immunogen Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
Klon 4E7
Isotyp IgG1
Plasmids, Primers & others

Target Details FEN1

Produktdetails anti-FEN1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-FEN1 Antikörper (ABIN151083) Bilder zurück nach oben
Antigen
Andere Bezeichnung FEN-1 (FEN1 Antibody Abstract)
Hintergrund Gene Symbol: FEN1
Molekulargewicht Theoretical MW: 42 kDa
Gen-ID 2237
UniProt P39748
Pathways Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA

Anwendungsinformationen

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Handhabung Referenzen für anti-FEN1 Antikörper (ABIN151083) Bilder zurück nach oben
Applikations-hinweise Western Blot 1:500-1:3000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500WB: Predicted molecular weight: 43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Referenzen für anti-FEN1 Antikörper (ABIN151083) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Konservierungs-mittel Without preservative
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-FEN1 Antikörper (ABIN151083)

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Jaiswal, Armas, Izumi, Strauss, Narayan: "Adenomatous polyposis coli interacts with flap endonuclease 1 to block its nuclear entry and function." in: Neoplasia (New York, N.Y.), Vol. 14, Issue 6, pp. 495-508, 2012 (Probematerial (Species): Human). Weitere Details: Western Blotting

Ma, Liang, Liu, Axcrona, Kvalheim, Stokke, Nesland, Suo: "Prostate cancer cell lines under hypoxia exhibit greater stem-like properties." in: PLoS ONE, Vol. 6, Issue 12, pp. e29170, 2012

Tann, Boldogh, Meiss, Qian, Van Houten, Mitra, Szczesny: "Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 31975-83, 2011 (Probematerial (Species): Human).

Jaiswal, Narayan: "Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity." in: Biochemistry, Vol. 50, Issue 11, pp. 1901-9, 2011

Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." in: DNA repair, Vol. 9, Issue 2, pp. 109-19, 2010 (Probematerial (Species): Human).

Guo, Zheng, Xu, Dai, Zhou, Pascua, Chen, Shen: "Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding." in: Nature chemical biology, Vol. 6, Issue 10, pp. 766-73, 2010 (Probematerial (Species): Human).

Guo, Zheng, Dai, Zhou, Xu, Shen: "Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity." in: Nucleic acids research, Vol. 37, Issue 10, pp. 3431-41, 2009 (Probematerial (Species): Human).

Kong, Mohanty, Stephens, Heale, Gomez-Godinez, Shi, Kim, Yokomori, Berns: "Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells." in: Nucleic acids research, Vol. 37, Issue 9, pp. e68, 2009 (Probematerial (Species): Human).

Guo, Chavez, Singh, Finger, Hang, Hegde, Shen: "Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways." in: Journal of molecular biology, Vol. 377, Issue 3, pp. 679-90, 2008 (Probematerial (Species): Human).

Guo, Qian, Liu, Dai, Zhou, Zheng, Shen: "Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair." in: Molecular and cellular biology, Vol. 28, Issue 13, pp. 4310-9, 2008 (Probematerial (Species): Human).

Liu, Qian, Sung, de Souza-Pinto, Zheng, Bogenhagen, Bohr, Wilson, Shen, Demple: "Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria." in: Molecular and cellular biology, Vol. 28, Issue 16, pp. 4975-87, 2008 (Probematerial (Species): Human).

Szczesny, Tann, Longley, Copeland, Mitra: "Long patch base excision repair in mammalian mitochondrial genomes." in: The Journal of biological chemistry, Vol. 283, Issue 39, pp. 26349-56, 2008 (Probematerial (Species): Human).

Prasad, Liu, Deterding, Poltoratsky, Kedar, Horton, Kanno, Asagoshi, Hou, Khodyreva, Lavrik, Tomer, Yasui, Wilson: "HMGB1 is a cofactor in mammalian base excision repair." in: Molecular cell, Vol. 27, Issue 5, pp. 829-41, 2007 (Probematerial (Species): Human).

Kundu, Balusu, Jaiswal, Narayan: "Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways." in: Carcinogenesis, Vol. 28, Issue 10, pp. 2089-95, 2007

Shibata, Nakamura: "Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 746-54, 2002 (Probematerial (Species): Human).

Kedar, Kim, Robertson, Hou, Prasad, Horton, Wilson: "Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31115-23, 2002 (Probematerial (Species): Human).

Qiu, Li, Frank, Shen: "Cell cycle-dependent and DNA damage-inducible nuclear localization of FEN-1 nuclease is consistent with its dual functions in DNA replication and repair." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4901-8, 2001

Bilder

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Handhabung Referenzen für anti-FEN1 Antikörper (ABIN151083) zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibody (ABIN151083) Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4E7) [ABIN151083] - Analysis ...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibody (ABIN151083) Western Blot: FEN-1 Antibody (4E7) [ABIN151083] - A: MCF7.
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibody (ABIN151083) Western Blot: FEN-1 Antibody (4E7) [ABIN151083] - Whole cell extract (30 ug) was sepa...