anti-Human FEN1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended FEN1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Flap Structure-Specific Endonuclease 1 (FEN1) Antikörper
  • FEN-1
  • FEN1
  • AW538437
  • MF1
  • RAD2
  • cb879
  • fen-1
  • fen1
  • mf1
  • rad2
  • xFEN1a
  • flap structure-specific endonuclease 1
  • flap endonuclease 1-like
  • Flap endonuclease-1
  • 5'-3' exonuclease family protein
  • flap structure specific endonuclease 1
  • flap structure-specific endonuclease 1 L homeolog
  • nuclease, Rad2 family
  • FEN1
  • LOC100808044
  • Bm1_49605
  • AT5G26680
  • Fen1
  • fen1
  • fen1.L
Epitop
Center
16
8
7
7
6
6
4
4
3
2
2
2
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
144
84
56
7
7
6
6
6
4
4
4
3
1
1
1
1
Wirt
Kaninchen
93
51
Klonalität
Polyklonal
Konjugat
Dieser FEN1 Antikörper ist unkonjugiert
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
124
43
38
29
28
20
18
13
8
4
3
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN2855166
$ 466.18
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
20.474512 ABIN151787 ICC IF IHC IHC (p) IP WB Rabbit AA 250-300 Anmelden zum Anzeigen Polyclonal 6
13 ABIN2681984 IF IF/ICC IHC (p) IP WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
11.474512 ABIN4311324 ICC IF IHC IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
8.494791 ABIN2740837 IF IHC IHC (p) IP WB Rabbit IgG AA 250-300 Anmelden zum Anzeigen Polyclonal 0
7 ABIN272186 IF IHC (p) WB Rabbit Anmelden zum Anzeigen Polyclonal 0
7 ABIN5774499 IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
4 ABIN1387673 IF (p) IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
4 ABIN5578042 IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 4E7 0
1 ABIN2620777 IHC IHC (p) WB Mouse IgG1 AA 117-380 Anmelden zum Anzeigen 1F3 0
1 ABIN2620778 IHC IHC (p) WB Mouse IgG1 AA 117-380 Anmelden zum Anzeigen 3A8 0
1 ABIN2892105 IF IHC IHC (p) WB Rabbit Ala119 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5499244 IHC IHC (p) WB Mouse IgG1 AA 1-380 Anmelden zum Anzeigen 4E7 0
1 ABIN2620779 ICC IF IHC IHC (p) IP WB Rabbit Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN1423054 IHC (p) WB HRP Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1895546 IF IHC IHC (p) Rabbit IgG AA 250-300 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1405183 IHC (p) WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN5959480 ELISA IF IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN2962739 IHC (p) WB Mouse IgG1 AA 1-380, full length Anmelden zum Anzeigen (4E7) 0

General

Antigen Flap Structure-Specific Endonuclease 1 (FEN1) Antikörper
Epitop Center
(16), (8), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(144), (84), (56), (7), (7), (6), (6), (6), (4), (4), (4), (3), (1), (1), (1), (1)
Wirt Kaninchen
(93), (51)
Klonalität
Polyklonal
Konjugat Dieser FEN1 Antikörper ist unkonjugiert
(2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(124), (43), (38), (29), (28), (20), (18), (13), (8), (4), (3), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FEN1 Antikörper

Target Details FEN1 Anwendungsinformationen Handhabung Bilder
Produktmerkmale Rabbit Polyclonal antibody to FEN1 (flap structure-specific endonuclease 1)
FEN1 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.
Isotyp IgG
Plasmids, Primers & others

Target Details FEN1

Produktdetails anti-FEN1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung FEN1 (FEN1 Antibody Abstract)
Hintergrund The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Cellular Localization: Nucleus
Molekulargewicht 43 kDa
Gen-ID 2237
Pathways Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA

Anwendungsinformationen

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Handhabung Bilder zurück nach oben
Applikations-hinweise Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Kommentare

Positive Control: HepG2 , NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.72 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungs-mittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-FEN1 Antikörper Target Details FEN1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A549, using FEN1, ...
Immunoprecipitation (IP) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) IP Image FEN1 antibody immunoprecipitates FEN1 protein in IP experiments. IP samples:...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) WB Image Sample (30 ug of whole cell lysate) A: Hep G2 , 10% SDS PAGE antibody dilute...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) WB Image FEN1 antibody detects FEN1 protein by Western blot analysis. A. 30 µg PC-12 ...
Western Blotting (WB) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) WB Image FEN1 antibody detects FEN1 protein by Western blot analysis. A. 30 µg NIH-3T...
Immunohistochemistry (IHC) image for anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Center) antibody (ABIN2855166) IHC-P Image FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma b...