anti-Human CD86 Antikörper für Immunoprecipitation

Recommended CD86 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD86 Molecule (CD86) Antikörper
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reaktivität
Human
462
412
181
10
6
3
2
2
1
1
Wirt
Maus
378
311
110
1
Klonalität
Monoklonal
Konjugat
Dieser CD86 Antikörper ist unkonjugiert
99
96
55
49
21
15
13
11
10
9
9
8
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
614
249
179
176
133
96
88
69
54
54
43
16
13
13
12
11
8
2
2
2
2
1
1
1
1
1
Optionen
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Bilder

Produktnummer ABIN302084
$ 366.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
24.760653 ABIN302014 FACS Func IHC (f) IP WB FITC Mouse IgG1 Anmelden zum Anzeigen BU63 9
24.760653 ABIN301986 FACS Func IHC (f) IP WB APC Mouse IgG1 Anmelden zum Anzeigen BU63 9
24.760653 ABIN302058 FACS Func IHC (f) IP WB PerCP Mouse IgG1 Anmelden zum Anzeigen BU63 9
24.760653 ABIN302037 FACS Func IHC (f) IP WB PE Mouse IgG1 Anmelden zum Anzeigen BU63 9
24.760653 ABIN2749230 Func IHC (f) FACS IP WB Mouse IgG1 Anmelden zum Anzeigen BU63 9
15.760653 ABIN2144699 IHC (f) FACS IP WB FITC Mouse IgG1 Anmelden zum Anzeigen 4ABU63 4
15.760653 ABIN2144701 IHC (f) FACS IP WB FITC Mouse IgG1 Anmelden zum Anzeigen 4ABU63 4
15.760653 ABIN2144981 IHC (f) FACS IP WB PE Mouse IgG1 Anmelden zum Anzeigen 4ABU63 4
15.760653 ABIN2144984 IHC (f) FACS IP WB PE Mouse IgG1 Anmelden zum Anzeigen 4ABU63 4
13.204659 ABIN395963 IP Mouse IgG2a kappa AA 220-329 Anmelden zum Anzeigen 3D7 0
13.204659 ABIN560238 IP ELISA Mouse IgG2a kappa AA 220-329, partial Anmelden zum Anzeigen 3D7 0
12.760653 ABIN234755 IP Mouse IgG1 Anmelden zum Anzeigen BU63 0
12.760653 ABIN2137059 Func FACS IP IHC Mouse IgG2b kappa Anmelden zum Anzeigen IT2-2 0
10 ABIN1871657 ICC IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
10 ABIN2895521 IF/ICC IHC IP WB Rabbit AA 46-282 Anmelden zum Anzeigen Polyclonal 0
8.5 ABIN2472677 IHC (fro) FACS IP Mouse IgG1 Anmelden zum Anzeigen BU63 4
7 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Anmelden zum Anzeigen BU63 0
7 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Anmelden zum Anzeigen BU63 0
7 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Anmelden zum Anzeigen BU63 0
7 ABIN6185431 FACS IF IHC (f) IP WB Mouse IgG1, kappa Anmelden zum Anzeigen BU63 0

General

Antigen CD86 Molecule (CD86) Antikörper
Reaktivität Human
(462), (412), (181), (10), (6), (3), (2), (2), (1), (1)
Wirt Maus
(378), (311), (110), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD86 Antikörper ist unkonjugiert
(99), (96), (55), (49), (21), (15), (13), (11), (10), (9), (9), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(614), (249), (179), (176), (133), (96), (88), (69), (54), (54), (43), (16), (13), (13), (12), (11), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD86 Antikörper

Target Details CD86 Anwendungsinformationen Handhabung Referenzen für anti-CD86 Antikörper (ABIN302084) Bilder
Spezifität The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
HLDA V, WS Code BP BP072
HLDA V, WS Code A A109
HLDA VI, WS Code BP 95
HLDA VI, WS Code B CD86.9
Reinigung Purified from cell culture supernatant by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Immunogen B-lymphoblastoid cell line ARH 77
Klon BU63
Isotyp IgG1
Plasmids, Primers & others

Target Details CD86

Produktdetails anti-CD86 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD86 Antikörper (ABIN302084) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD86 (CD86 Antibody Abstract)
Hintergrund CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Anwendungsinformationen

Produktdetails anti-CD86 Antikörper Target Details CD86 Handhabung Referenzen für anti-CD86 Antikörper (ABIN302084) Bilder zurück nach oben
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD86 Antikörper Target Details CD86 Anwendungsinformationen Referenzen für anti-CD86 Antikörper (ABIN302084) Bilder zurück nach oben
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Referenzen für anti-CD86 Antikörper (ABIN302084)

Produktdetails anti-CD86 Antikörper Target Details CD86 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007

Chan, Baird, Mercer, Fleming: "Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3177-81, 2006

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, 2003

Mauri, Wyss-Coray, Gallati, Pichler: "Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 118-27, 1995

Caux, Vanbervliet, Massacrier, Azuma, Okumura, Lanier, Banchereau: "B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1841-7, 1994

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, 1994

Allgemeine Veröffentlichungen

Yadav, Judkowski, Flodstrom-Tullberg, Sterling, Redmond, Sherman, Sarvetnick: "B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3631-9, 2004

Vasilevko, Ghochikyan, Holterman, Agadjanyan: "CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA." in: DNA and cell biology, Vol. 21, Issue 3, pp. 137-49, 2002

Bilder

Produktdetails anti-CD86 Antikörper Target Details CD86 Anwendungsinformationen Handhabung Referenzen für anti-CD86 Antikörper (ABIN302084) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN302084) Surface staining of human peripheral blood cells with anti-CD86 (BU63) PerCP (monocyt...
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN302084) Surface staining of human peripheral blood with anti-human CD44 (MEM-85) purified / G...