anti-Hund CD80 Antikörper für Blocking Reagent

Recommended CD80 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD80 Antikörper
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Reaktivität
Hund, Maus
456
285
49
11
10
6
3
3
2
2
1
Wirt
Armenischer Hamster
399
126
97
94
41
16
Klonalität
Monoklonal
Konjugat
Dieser CD80 Antikörper ist unkonjugiert
71
70
53
40
19
17
16
15
10
7
7
6
6
6
6
6
6
6
6
5
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
508
220
171
153
114
101
81
42
39
26
22
22
12
8
7
6
6
5
5
5
2
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1176972
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
10 ABIN2689407 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Anmelden zum Anzeigen 16 6
1 ABIN1967873 BR FACS Func IHC IHC (fro) IP Neut Hamster IgG2 Anmelden zum Anzeigen 16-10A1 0

General

Antigen CD80 Antikörper
Reaktivität Hund, Maus
(456), (285), (49), (11), (10), (6), (3), (3), (2), (2), (1)
Wirt Armenischer Hamster
(399), (126), (97), (94), (41), (16)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD80 Antikörper ist unkonjugiert
(71), (70), (53), (40), (19), (17), (16), (15), (10), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(508), (220), (171), (153), (114), (101), (81), (42), (39), (26), (22), (22), (12), (8), (7), (6), (6), (5), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 6 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD80 Antikörper

Target Details CD80 Anwendungsinformationen Handhabung Referenzen für anti-CD80 Antikörper (ABIN1176972) Bilder
Marke BD Pharmingen™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterilität 0.2 μm filtered
Endotoxin-Niveau Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse CD80 (B7) Transfected Cell Line
Klon 16-10A1
Isotyp IgG2, kappa
Plasmids, Primers & others

Target Details CD80

Produktdetails anti-CD80 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD80 Antikörper (ABIN1176972) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD80 (CD80 Antibody Abstract)
Hintergrund The 16-10A1 antibody reacts with CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4). CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages, and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines. However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4). Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat. No. 553368). In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN-gamma-activated alveolar macrophages of the dog.
Synonyms: B7-1
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Immune Effector Process

Anwendungsinformationen

Produktdetails anti-CD80 Antikörper Target Details CD80 Handhabung Referenzen für anti-CD80 Antikörper (ABIN1176972) Bilder zurück nach oben
Applikations-hinweise Flow Cytometry: For flow cytometry of leukocytes, it is recommended that Mouse BD Fc Block™ (Cat. No. 553141) be used. When using Mouse BD Fc Block™ (clone 2.4G2), it is important that the second-step anti-hamster IgG antibody does not cross-react with the 2.4G2 mAb (rat IgG2b, kappa), we have found that the anti-hamster IgG cocktail (Cat. No. 554010) to be effective. Since this antigen is expressed at low density, it may be desirable to amplify staining by using a biotinylated second-step reagent followed by a bright third-step reagent, such as PE streptavidin (Cat. No. 554061). Expression of membrane CD80 by mouse splenocytes. Freshly isolated (left panel) or 72-hour LPS-stimulated BALB/c splenocytes (right panel) were pretreated with Mouse BD Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141) and stained with either purified 16-10A1 mAb (open histograms) or purified hamster IgG isotype control mAb (shaded histograms). Staining was detected with biotinylated mouse anti-hamster IgG (Cat. No. 554010) followed by PE streptavidin (Cat. No. 554061). Flow cytometry was performed on a FACScan™ (BD Biosciences, San Jose, CA).
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD80 Antikörper Target Details CD80 Anwendungsinformationen Referenzen für anti-CD80 Antikörper (ABIN1176972) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Konservierungs-mittel Azide free
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CD80 Antikörper (ABIN1176972)

Produktdetails anti-CD80 Antikörper Target Details CD80 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Sojka, Donepudi, Bluestone, Mokyr: "Melphalan and other anticancer modalities up-regulate B7-1 gene expression in tumor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 12, pp. 6230-6, 2000

Herold, Vezys, Koons, Lenschow, Thompson, Bluestone: "CD28/B7 costimulation regulates autoimmune diabetes induced with multiple low doses of streptozotocin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 984-91, 1997

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

Boussiotis, Gribben, Freeman, Nadler: "Blockade of the CD28 co-stimulatory pathway: a means to induce tolerance." in: Current opinion in immunology, Vol. 6, Issue 5, pp. 797-807, 1995

Hathcock, Laszlo, Pucillo, Linsley, Hodes: "Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 631-40, 1994

Razi-Wolf, Freeman, Galvin, Benacerraf, Nadler, Reiser: "Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 4210-4, 1992

Bilder

Produktdetails anti-CD80 Antikörper Target Details CD80 Anwendungsinformationen Handhabung Referenzen für anti-CD80 Antikörper (ABIN1176972) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD80 (CD80) antibody (ABIN1176972) anti-CD80 (CD80) antibody