anti-Maus CD28 Antikörper für Cytotoxicity Test

Recommended CD28 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD28 Antikörper
  • Tp44
  • CD28RNA
  • cd28
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • Cd28
  • cd28.L
Reaktivität
Maus
569
392
96
49
44
43
43
36
16
7
6
2
2
2
2
1
1
1
Wirt
Golden Syrian Hamster
556
170
99
59
50
31
12
1
Klonalität
Monoklonal
Konjugat
Dieser CD28 Antikörper ist unkonjugiert
111
91
72
58
22
19
13
13
11
10
9
9
9
9
8
8
8
8
8
8
8
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
Applikation
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
796
266
176
157
149
144
127
125
85
52
48
26
13
11
9
5
3
3
2
2
2
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Produktnummer ABIN1176978
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2689144 BR CyTox IHC (fro) FACS IP Golden Syrian Hamster IgG2, lambda Anmelden zum Anzeigen 37-51 13
1 ABIN2689145 BR CyTox IHC (fro) FACS IP Golden Syrian Hamster IgG2, lambda Anmelden zum Anzeigen 37-51 0

General

Antigen CD28 Antikörper
Reaktivität Maus
(569), (392), (96), (49), (44), (43), (43), (36), (16), (7), (6), (2), (2), (2), (2), (1), (1), (1)
Wirt Golden Syrian Hamster
(556), (170), (99), (59), (50), (31), (12), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD28 Antikörper ist unkonjugiert
(111), (91), (72), (58), (22), (19), (13), (13), (11), (10), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Applikation Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(796), (266), (176), (157), (149), (144), (127), (125), (85), (52), (48), (26), (13), (11), (9), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 14 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD28 Antikörper

Target Details CD28 Anwendungsinformationen Handhabung Referenzen für anti-CD28 Antikörper (ABIN1176978) Bilder
Marke BD Pharmingen™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterilität 0.2 μm filtered
Endotoxin-Niveau Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse EL-4 (T-cell lymphoma) Cells
Klon 37-51
Isotyp IgG2, lambda
Plasmids, Primers & others

Target Details CD28

Produktdetails anti-CD28 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD28 Antikörper (ABIN1176978) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD28 (CD28 Antibody Abstract)
Hintergrund The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor, its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Anwendungsinformationen

Produktdetails anti-CD28 Antikörper Target Details CD28 Handhabung Referenzen für anti-CD28 Antikörper (ABIN1176978) Bilder zurück nach oben
Applikations-hinweise Flow Cytometry: For multicolor staining of cell suspensions from peripheral lymphoid tissues (e.g detection of T lymphocytes and/or NK cells), investigators are encouraged to utilize Purified Rat Anti-Mouse CD16/CD32 [Mouse BD Fc Block™] (Cat. No. 553141) to minimize non-specific binding. In addition, since CD28 may be expressed at low density on resting peripheral T lymphocytes, investigators may wish to amplify the staining signal by using a biotinylated second-step antibody followed by a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061). If Mouse BD Fc Block™ is used, investigators need to be aware of and account for any potential crossreactivity that may occur between the second-step or third-step reagents chosen with the Mouse BD Fc Block™. Biotin Mouse Anti-Syrian Hamster IgG2 (Cat. No. 554029) would be a suggested second-step antibody for investigators to consider utilizing.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD28 Antikörper Target Details CD28 Anwendungsinformationen Referenzen für anti-CD28 Antikörper (ABIN1176978) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
Konservierungs-mittel Azide free
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-CD28 Antikörper (ABIN1176978)

Produktdetails anti-CD28 Antikörper Target Details CD28 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Marietta, Weis, Weis: "CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2840-8, 1997

Ong, Lim, Teh: "CD28-induced cytokine production and proliferation by thymocytes are differentially regulated by the p59fyn tyrosine kinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2169-76, 1997

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." in: International immunology, Vol. 9, Issue 1, pp. 161-7, 1997

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, 1995

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, 1994

Harding, Allison: "CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1791-6, 1993

Shahinian, Pfeffer, Lee, Kündig, Kishihara, Wakeham, Kawai, Ohashi, Thompson, Mak: "Differential T cell costimulatory requirements in CD28-deficient mice." in: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 609-12, 1993

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992