anti-Maus CD28 Antikörper für Cytotoxicity Test

Recommended CD28 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
CD28 Antikörper
  • Tp44
  • CAE2
  • ECA2
  • GEFSP3
  • CD28RNA
  • cd28
  • CD28 molecule
  • gamma-aminobutyric acid type A receptor gamma2 subunit
  • CD28 antigen
  • Cd28 molecule
  • CD28 molecule L homeolog
  • CD28
  • GABRG2
  • Cd28
  • cd28.L
Reaktivität
Maus
598
444
97
70
67
61
61
44
34
27
16
7
2
2
2
2
1
1
1
Wirt
Golden Syrian Hamster
617
190
101
66
57
31
12
1
Klonalität
Monoklonal
Konjugat
Dieser CD28 Antikörper ist unkonjugiert
116
96
78
62
26
22
19
18
17
15
12
12
12
12
12
12
11
10
10
9
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
Applikation
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
888
268
193
191
155
151
146
130
85
70
48
26
13
12
9
8
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN2689144
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1176978 BR CyTox IHC (fro) FACS IP Golden Syrian Hamster IgG2, lambda Anmelden zum Anzeigen 37-51 14
1 ABIN2689145 BR CyTox IHC (fro) FACS IP Golden Syrian Hamster IgG2, lambda Anmelden zum Anzeigen 37-51 0

General

Antigen CD28 Antikörper
Reaktivität Maus
(598), (444), (97), (70), (67), (61), (61), (44), (34), (27), (16), (7), (2), (2), (2), (2), (1), (1), (1)
Wirt Golden Syrian Hamster
(617), (190), (101), (66), (57), (31), (12), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser CD28 Antikörper ist unkonjugiert
(116), (96), (78), (62), (26), (22), (19), (18), (17), (15), (12), (12), (12), (12), (12), (12), (11), (10), (10), (9), (9), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Applikation Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(888), (268), (193), (191), (155), (151), (146), (130), (85), (70), (48), (26), (13), (12), (9), (8), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 13 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CD28 Antikörper

Target Details CD28 Anwendungsinformationen Handhabung Referenzen für anti-CD28 Antikörper (ABIN2689144) Bilder
Marke BD Pharmingen™
Produktmerkmale The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor, its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of CD28 expression on splenic T lymphocytes. BALB/c splenocytes were preincubated with purified anti-mouse CD16/CD32 mAb [Mouse BD Fc Block™] (Cat. No. 553141), then stained simultaneously with FITC-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553046) and FITC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553030) in addition to staining with purified anti-mouse CD28 clone 37.51 (right panel). Staining of CD28-positive cells was detected with biotinylated anti-Syrian hamster IgG2 mAb G192-3 (Cat. No. 554029), followed by streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Hamster Anti-Mouse CD28 - Purified - Clone 37.51 - Isotype Syrian Hamster IgG2, λ1 - Reactivity Ms - 0.1 mg
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse EL-4 (T-cell lymphoma) Cells
Klon 37-51
Isotyp IgG2, lambda
Plasmids, Primers & others

Target Details CD28

Produktdetails anti-CD28 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-CD28 Antikörper (ABIN2689144) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD28 (CD28 Antibody Abstract)
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Anwendungsinformationen

Produktdetails anti-CD28 Antikörper Target Details CD28 Handhabung Referenzen für anti-CD28 Antikörper (ABIN2689144) Bilder zurück nach oben
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CD28 Antikörper Target Details CD28 Anwendungsinformationen Referenzen für anti-CD28 Antikörper (ABIN2689144) Bilder zurück nach oben
Konzentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C.

Referenzen für anti-CD28 Antikörper (ABIN2689144)

Produktdetails anti-CD28 Antikörper Target Details CD28 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Marietta, Weis, Weis: "CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2840-8, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." in: International immunology, Vol. 9, Issue 1, pp. 161-7, 1997

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, 1995

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, 1994

Harding, Allison: "CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1791-6, 1993

Shahinian, Pfeffer, Lee, Kündig, Kishihara, Wakeham, Kawai, Ohashi, Thompson, Mak: "Differential T cell costimulatory requirements in CD28-deficient mice." in: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 609-12, 1993

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992