|Antigen||gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3) Antikörper|
|Epitop||AA 370-433 Alternativen|
|Reaktivität||Human, Maus, Ratte (Rattus) Alternativen|
|Konjugat||Dieser GABRB3 Antikörper ist unkonjugiert Alternativen|
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|Hersteller||Anmelden zum Anzeigen|
Produktdetails anti-GABRB3 AntikörperTarget Details GABRB3 Anwendungsinformationen Handhabung Bilder
|Spezifität||Detects ~55 kDa protein. No cross-reactivity against GABAA Receptor Beta 2 or GABAA Receptor Beta 1.|
|Produktmerkmale||Synonyms: GABRB-3, GABA A receptor subunit beta-3, Gamma-aminobutyric acid receptor subunitbeta-3|
|Reinigung||Protein G Chromatography.|
|Immunogen||Fusion protein amino acids 370-433 of Mouse GABA-A-R-Beta3 (accession numberAAB60502).|
Target Details GABRB3Produktdetails anti-GABRB3 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
|Andere Bezeichnung||GABRB3 (GABRB3 Antibody Abstract)|
|Hintergrund||Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).Synonyms: GABA A receptor subunit beta-3, GABRB-3, Gamma-aminobutyric acid receptor subunit beta-3|
|Pathways||Sensory Perception of Sound|
AnwendungsinformationenProduktdetails anti-GABRB3 Antikörper Target Details GABRB3 Handhabung Bilder zurück nach oben
Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Beta3 GABA Receptor in 10 μg of Rat brain lysate bycolorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondaryantibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails anti-GABRB3 Antikörper Target Details GABRB3 Anwendungsinformationen Bilder zurück nach oben
|Buffer||PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.|
|Vorsichtsmaßnahmen||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Lagerung||4 °C/-20 °C|
|Informationen zur Lagerung||
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.