Maus E-cadherin ELISA Kit

Recommended E-cadherin Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) ELISA Kits
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • arc-1
  • cd324
  • cdhe
  • ecad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • cadherin 1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin 1, type 1 S homeolog
  • CDH1
  • cdh1
  • Cdh1
  • cdh1.S
Epitop
AA 157-709
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
34
25
12
4
4
3
2
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
156-10000 pg/mL
Untere Nachweisgrenze
156 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN921078
$ 430.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.739196 ABIN1568917 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Anmelden zum Anzeigen
10.739196 ABIN1113873 Sandwich ELISA Plasma, Serum 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.739196 ABIN625120 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 6-3000 pg/mL 6 pg/mL Anmelden zum Anzeigen
10.739196 ABIN2708500 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Anmelden zum Anzeigen
10.739196 ABIN2113317 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Tissue Homogenate Anmelden zum Anzeigen
1 ABIN367706 Sandwich ELISA Serum, Plasma, Tissue Homogenate Anmelden zum Anzeigen
1 ABIN627794 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate 0.5-10 ng/mL 0.5 ng/mL Anmelden zum Anzeigen
1 ABIN4993054 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-3000 pg/mL 4.1 pg/mL Anmelden zum Anzeigen
1 ABIN5521247 Sandwich ELISA Plasma, Serum, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN1112588 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Anmelden zum Anzeigen
1 ABIN5652447 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1 pg/mL - 3000 pg/mL 4.1 pg/mL Anmelden zum Anzeigen
1 ABIN4967654 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Anmelden zum Anzeigen
1 ABIN4885722 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Anmelden zum Anzeigen
1 ABIN2683767 Sandwich ELISA Cell Culture Supernatant, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Anmelden zum Anzeigen
1 ABIN2683768 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Anmelden zum Anzeigen
1 ABIN1846901 Tissue Homogenate 156-10000 pg/mL 156 pg/mL Anmelden zum Anzeigen
1 ABIN2042441 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Anmelden zum Anzeigen
1 ABIN5670125 Sandwich ELISA 15.625 pg/mL - 1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2543972 Tissue Homogenate 30-1500 pg/mL 30 pg/mL Anmelden zum Anzeigen
1 ABIN5072695 Sandwich ELISA Plasma, Serum, Tissue Homogenate Anmelden zum Anzeigen

General

Antigen Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) ELISA Kits
Epitop AA 157-709
Reaktivität Maus
Kits mit alternativen Reaktivitäten:
(34), (25), (12), (4), (4), (3), (2), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 156-10000 pg/mL
Untere Nachweisgrenze 156 pg/mL
Applikation ELISA
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails E-cadherin ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für E-cadherin Kit (ABIN921078) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse E-Cadherin
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: NSO
Immunogen sequence: D157-V709
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <15pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: D157-V709

Antigendetails

Produktdetails E-cadherin ELISA Kit Anwendungsinformationen Handhabung Referenzen für E-cadherin Kit (ABIN921078) Bilder zurück nach oben
Antigen
Andere Bezeichnung CDH1 (CDH1 ELISA Kit Abstract)
Hintergrund

Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity). .

Background: E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.

Synonyms: Cadherin-1,ARC-1,Epithelial cadherin,E-cadherin,Uvomorulin,CD324,E-Cad/CTF1,E-Cad/CTF2,E-Cad/CTF3,Cdh1,

Full Gene Name: Cadherin-1

Cellular Localisation: Cell junction. Cell membrane, Single-pass type I membrane protein. Endosome . Golgi apparatus, trans-Golgi network . Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity)..
Gen-ID 12550
UniProt P09803
Forschungsgebiet Proteolysis / Ubiquitin
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation

Anwendungsinformationen

Produktdetails E-cadherin ELISA Kit Antigendetails Handhabung Referenzen für E-cadherin Kit (ABIN921078) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Non-neural epithelial tissues.

Plattentyp Pre-coated
Protokoll mouse E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for E-Cadherin has been precoated onto 96-well plates. Standards(NSO, D157-V709) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse E-Cadherin amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse E-Cadherin standard solution and each sample is measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(ng/ml): 1.09, Standard deviation: 0.062, CV(%): 5.7
  • Sample 2: n=16, Mean(ng/ml): 3.31, Standard deviation: 0.209, CV(%): 6.3
  • Sample 3: n=16, Mean(ng/ml): 6.76, Standard deviation: 0.487, CV(%): 7.2,
  • Sample 1: n=24, Mean(ng/ml): 1.14, Standard deviation: 0.072, CV(%): 6.3
  • Sample 2: n=24, Mean(ng/ml): 3.59, Standard deviation: 0.255, CV(%): 7.1
  • Sample 3: n=24, Mean(ng/ml): 7.23, Standard deviation: 0.528, CV(%): 7.3
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails E-cadherin ELISA Kit Antigendetails Anwendungsinformationen Referenzen für E-cadherin Kit (ABIN921078) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen für E-cadherin Kit (ABIN921078)

Produktdetails E-cadherin ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Jin, Guo, Che, Sun, Liu, Zhang: "Germline stem cell lineage tracing by free-floating immunofluorescent assay of mouse seminiferous tubule." in: Microscopy research and technique, Vol. 75, Issue 8, pp. 1150-3, 2012

Batlle, Sancho, Francí, Domínguez, Monfar, Baulida, García De Herreros: "The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells." in: Nature cell biology, Vol. 2, Issue 2, pp. 84-9, 2000

Berx, Staes, van Hengel, Molemans, Bussemakers, van Bokhoven, van Roy: "Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1)." in: Genomics, Vol. 26, Issue 2, pp. 281-9, 1995

Becker, Atkinson, Reich, Becker, Nekarda, Siewert, Höfler: "E-cadherin gene mutations provide clues to diffuse type gastric carcinomas." in: Cancer research, Vol. 54, Issue 14, pp. 3845-52, 1994

Bilder

Produktdetails E-cadherin ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für E-cadherin Kit (ABIN921078) zurück nach oben
Bilder des Herstellers
ELISA image for Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) ELISA Kit (ABIN921078) Mouse E-Cadherin PicoKine ELISA Kit standard curve