anti-Human MTOR Antikörper für Immunofluorescence

Recommended MTOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
C-Term
64
36
28
21
19
15
15
13
12
10
8
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
367
195
192
18
9
8
7
7
7
7
4
4
2
1
1
Wirt
Kaninchen
316
47
3
1
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
251
114
100
71
54
52
34
19
8
7
7
6
3
2
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2855088
$ 358.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.739032 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Anmelden zum Anzeigen Polyclonal 4
11.739032 ABIN2705013 IC IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
11.739032 ABIN1531447 IF IHC ELISA Rabbit IgG AA 2447-2496, pSer2481 Anmelden zum Anzeigen Polyclonal 0
11.739032 ABIN968383 IF WB Mouse IgG1 AA 185-290 Anmelden zum Anzeigen 30-RAFT1 5
11.739032 ABIN3031742 IF IHC ELISA WB Rabbit Ig Fraction AA 2459-2488 Anmelden zum Anzeigen Polyclonal 0
1 ABIN968384 IF WB Mouse IgG1 AA 185-290 Anmelden zum Anzeigen 30-RAFT1 5
1 ABIN390217 IF IHC (p) WB Rabbit Ig Fraction AA 2459-2488, Ser2481 Anmelden zum Anzeigen Polyclonal 1
1 ABIN196962 IF IHC (p) WB Rabbit pSer2448 Anmelden zum Anzeigen Polyclonal 4
1 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 1
1 ABIN2755575 IC IF IP IHC WB Rabbit C-Term, pSer2448 Anmelden zum Anzeigen Polyclonal 0
1 ABIN319329 IF IHC (p) WB Rabbit Ser2448 Anmelden zum Anzeigen Polyclonal 3
1 ABIN1870445 IF IHC WB Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal 0
1 ABIN214561 IF IHC (p) ELISA WB Rabbit AA 2440-2457 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1737123 IF IHC (p) ELISA WB Rabbit IgG AA 2440-2457 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2879300 IF IHC (p) WB Rabbit pSer2442 Anmelden zum Anzeigen Polyclonal 0
1 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Anmelden zum Anzeigen Polyclonal 0
1 ABIN5555365 EIA IF WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Anmelden zum Anzeigen Polyclonal 0
1 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal 0
1 ABIN3183004 ELISA IF IHC Rabbit IgG pSer2481 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Epitop C-Term
(64), (36), (28), (21), (19), (15), (15), (13), (12), (10), (8), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(367), (195), (192), (18), (9), (8), (7), (7), (7), (7), (4), (4), (2), (1), (1)
Wirt Kaninchen
(316), (47), (3), (1)
Klonalität
Polyklonal
Konjugat Dieser MTOR Antikörper ist unkonjugiert
(8), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(251), (114), (100), (71), (54), (52), (34), (19), (8), (7), (7), (6), (3), (2), (2), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MTOR Antikörper

Target Details MTOR Anwendungsinformationen Handhabung Bilder
Produktmerkmale Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
Isotyp IgG

Target Details MTOR

Produktdetails anti-MTOR Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung mTOR (FRAP1 Antibody Abstract)
Hintergrund The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side
Molekulargewicht 289 kDa
Gen-ID 2475
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-MTOR Antikörper Target Details MTOR Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Kommentare

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Konservierungsmittel ProClin
Vorsichtsmaßnahmen This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) ICC/IF Image mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunoflu...
Immunoprecipitation (IP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IP Image Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse ...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immun...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue ex...
Immunoprecipitation (IP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IP Image mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) ChIP Image Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of ei...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Various whole c...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse tes...