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|Antigen||Epiregulin (EREG) ELISA Kits|
Kits mit alternativen Reaktivitäten:
|Detektionsbereich||15.6 pg/mL - 1000 pg/mL|
|Untere Nachweisgrenze||15.6 pg/mL|
|6 Publikationen vorhanden|
|Hersteller||Anmelden zum Anzeigen|
Produktdetails Epiregulin ELISA KitAntigendetails Anwendungsinformationen Handhabung Referenzen für Epiregulin Kit (ABIN415106) Bilder
|Verwendungszweck||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EREG in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids|
|Proben||Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate|
This assay has high sensitivity and excellent specificity for detection of Epiregulin (EREG).
|Kreuzreaktivität (Details)||No significant cross-reactivity or interference between Epiregulin (EREG) and analogues was observed.|
AntigendetailsProduktdetails Epiregulin ELISA Kit Anwendungsinformationen Handhabung Referenzen für Epiregulin Kit (ABIN415106) Bilder zurück nach oben
|Andere Bezeichnung||EREG (EREG ELISA Kit Abstract)|
|Pathways||RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation|
AnwendungsinformationenProduktdetails Epiregulin ELISA Kit Antigendetails Handhabung Referenzen für Epiregulin Kit (ABIN415106) Bilder zurück nach oben
Information on standard material:
|Protokoll||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epiregulin (EREG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epiregulin (EREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epiregulin (EREG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Epiregulin (EREG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
|Aufbereitung der Reagenzien||
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Aufbereitung der Proben||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Create a standard curve on log-log graph paper, with EREG concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epiregulin (EREG) were tested 20 times on one plate, respectively.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails Epiregulin ELISA Kit Antigendetails Anwendungsinformationen Referenzen für Epiregulin Kit (ABIN415106) Bilder zurück nach oben
|Vorsichtsmaßnahmen||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Informationen zur Lagerung||
Referenzen für Epiregulin Kit (ABIN415106)Produktdetails Epiregulin ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
|Produkt verwendet in:||
Rizzi, Migliario, Rocchetti, Tonello, Renò: "Epiregulin-loaded PLGA nanoparticles increase human keratinocytes proliferation: preliminary data." in: European review for medical and pharmacological sciences, Vol. 20, Issue 12, pp. 2484-90, 2016
John, Lahoti, Wagner, Hughes, Perdew: "The Ah receptor regulates growth factor expression in head and neck squamous cell carcinoma cell lines." in: Molecular carcinogenesis, Vol. 53, Issue 10, pp. 765-76, 2014
Tomita, Haga, Mizuno, Katsumi, Sato, Okumoto, Nishise, Watanabe, Saito, Ueno: "Epiregulin promotes the emergence and proliferation of adult liver progenitor cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 307, Issue 1, pp. G50-7, 2014
Lahoti, Hughes, Kusnadi, John, Zhu, Murray, Gowda, Peters, Amin, Perdew: "Aryl hydrocarbon receptor antagonism attenuates growth factor expression, proliferation, and migration in fibroblast-like synoviocytes from patients with rheumatoid arthritis." in: The Journal of pharmacology and experimental therapeutics, Vol. 348, Issue 2, pp. 236-45, 2013
Oliveras-Ferraros, Cufí, Queralt, Vazquez-Martin, Martin-Castillo, de Llorens, Bosch-Barrera, Brunet, Menendez: "Cross-suppression of EGFR ligands amphiregulin and epiregulin and de-repression of FGFR3 signalling contribute to cetuximab resistance in wild-type KRAS tumour cells." in: British journal of cancer, Vol. 106, Issue 8, pp. 1406-14, 2012
Tao, Lu, Jiang, Qian, Wang, Gao, Jonas: "Apelin in plasma and vitreous and in fibrovascular retinal membranes of patients with proliferative diabetic retinopathy." in: Investigative ophthalmology & visual science, Vol. 51, Issue 8, pp. 4237-42, 2010
BilderProduktdetails Epiregulin ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für Epiregulin Kit (ABIN415106) zurück nach oben
|Bilder des Herstellers||