anti-Human Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa Antikörper für Immunofluorescence

Recommended Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa Antibody

Antigen
Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) Antikörper
  • Pol2rb
  • Rpb140
  • Rpb2
  • POL2RB
  • RPB2
  • hRPB140
  • hsRPB2
  • RNA polymerase II subunit B
  • polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
  • DNA-directed RNA polymerase II subunit RPB2
  • polymerase (RNA) II (DNA directed) polypeptide B
  • polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog
  • POLR2B
  • polr2b
  • LOC100640211
  • Polr2b
  • polr2b.L
  • rpb-2
Epitop
N-Term
10
8
8
7
3
3
1
1
1
1
Reaktivität
Human, Maus
47
19
18
8
7
4
4
4
3
3
3
2
2
Wirt
Kaninchen
47
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
47
21
13
4
3
2
2
1
Optionen

Bilder

Produktnummer ABIN2855420
$ 466.18
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
13.592318 ABIN5621938 ICC IF IHC (p) WB Rabbit IgG Polyclonal 0
10 ABIN5585931 IF IHC (p) WB Rabbit IgG Polyclonal 0
1 ABIN1885622 IF IHC WB Rabbit AA 1-142 Polyclonal 0
0.50146914 ABIN4351013 ICC IF IHC IHC (p) WB Rabbit IgG Polyclonal 0
-13.110054 ABIN4963453 ICC IF IHC IHC (p) WB Rabbit N-Term Polyclonal 0

General

Antigen Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) Antikörper
Epitop N-Term
(10), (8), (8), (7), (3), (3), (1), (1), (1), (1)
Reaktivität Human, Maus
(47), (19), (18), (8), (7), (4), (4), (4), (3), (3), (3), (2), (2)
Wirt Kaninchen
(47)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(2), (2), (2), (2), (2), (2)
Applikation Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(47), (21), (13), (4), (3), (2), (2), (1)
Hersteller

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Ratte (Rattus), Rhesusaffen, Rind (Kuh)
Kreuzreaktivität (Details) Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)
Produktmerkmale Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
Isotyp IgG

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung RPB2 (POLR2B Antibody Abstract)
Hintergrund This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Cellular Localization: Nucleus
Molekulargewicht 134 kDa
Gen-ID 5431
Pathways Regulatorische RNA Pathways, DNA Reparatur

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikations-hinweise Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Kommentare

Positive Control: H1299 , BCL-1

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.65 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungs-mittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPB2, ...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at...
Immunohistochemistry (IHC) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) IHC-P Image Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using...
Western Blotting (WB) image for anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibody (ABIN2855420) WB Image Sample (30 ug of whole cell lysate) A: BCL-1 5% SDS PAGE antibody diluted at...