anti-Ratte (Rattus) TACR1 Antikörper für Immunohistochemistry (Frozen Sections)

Recommended TACR1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Tachykinin Receptor 1 (TACR1) Antikörper
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Nk1r
  • Spr
  • Tac1r
  • nk1R
  • Tacr1
  • ASPR
  • tachykinin receptor 1
  • TACR1
  • Tacr1
Epitop
C-Term
23
18
15
14
11
8
7
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Meerschweinchen, Human, Maus, Ratte (Rattus)
150
71
64
10
10
6
5
5
4
4
2
2
1
1
1
1
Wirt
Kaninchen
136
16
9
Klonalität
Polyklonal
Konjugat
Dieser TACR1 Antikörper ist unkonjugiert
8
8
7
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
108
59
57
32
25
19
17
13
5
5
2
2
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN152451
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN307532 IHC IHC (fro) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN307533 ICC IHC IHC (fro) Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN326021 IHC IHC (fro) Rabbit IgG AA 385-407 Anmelden zum Anzeigen Polyclonal 0
1 ABIN307534 IHC IHC (fro) Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1737299 IHC (fro) IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Tachykinin Receptor 1 (TACR1) Antikörper
Epitop C-Term
(23), (18), (15), (14), (11), (8), (7), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Meerschweinchen, Human, Maus, Ratte (Rattus)
(150), (71), (64), (10), (10), (6), (5), (5), (4), (4), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(136), (16), (9)
Klonalität
Polyklonal
Konjugat Dieser TACR1 Antikörper ist unkonjugiert
(8), (8), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(108), (59), (57), (32), (25), (19), (17), (13), (5), (5), (2), (2), (1)
Pubmed 31 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TACR1 Antikörper

Target Details TACR1 Anwendungsinformationen Handhabung Referenzen für anti-TACR1 Antikörper (ABIN152451) Bilder
Spezifität This antibody is specific to the NK-1 receptor. Staining of cells transfected with NK-1 was abolished by preabsorption of the antisera with NK-1. Specificity was also verified by the localization of immunohistochemical staining with NB300-101 in neurons in
Reinigung Unpurified
Immunogen A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. [UniProt# P14600]
Plasmids, Primers & others

Target Details TACR1

Produktdetails anti-TACR1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TACR1 Antikörper (ABIN152451) Bilder zurück nach oben
Antigen
Andere Bezeichnung NK1R (TACR1 Antibody Abstract)
Hintergrund Gene Symbol: TACR1
Gen-ID 6869
UniProt P14600
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration

Anwendungsinformationen

Produktdetails anti-TACR1 Antikörper Target Details TACR1 Handhabung Referenzen für anti-TACR1 Antikörper (ABIN152451) Bilder zurück nach oben
Applikations-hinweise Western Blot 1:100-1:2000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:1000This Neurokinin 1 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. For ICC/IF staining, please see customer the review. Use in Immunohistochemistry on paraffin embedded sections was reported in scientific literature (PMID: 23792204).
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Neurokinin 1 Receptor Antibody Immunohistochemistry1) Perfuse rat through the ascending aorta with 500 mL. of 0.1 M phosphate buffered saline (PBS) (pH 7.4 at 4 degrees C), followed by 750 mL. of PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9, 4 degrees C). Tissue of interest is then dissected and post-fixed in PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9 at 4 degrees C) for at least 4 hours, followed by fixation in PBS containing 30 % sucrose (pH 7.4, 4 degrees C) for at least 24 hours. Until the tissue sinks.
2) Cut fixed sections at 60 um using a sliding microtome. 3) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Place tissue section in microcentrifuge tubes containing 1 mL. Of PBS, pH 7.4 as they are being cut. Wash sections (in PBS) on an upright rotator for 10-15 minutes.
) Remove PBS and add 1 mL. of blocking solution (PBS + 1 % normal donkey serum (NDS) + 0.3 % triton X-100).
) Incubate tissue sections in blocking solution for 30 minutes at room temperature (RT) on rotator. 7) Remove blocking solution and add anti-neurokinin-1 receptor or anti-Neurokinin-3 receptor antibody +PBS + 1 % NDS + 0.3 % triton X-100 + NB 300-101/102 at 1:100~1:10008) Incubate overnight on rotator at RT.
) Remove primary antisera and perform 3 x 10 minute PBS washes (1ml volume) on rotator.
) Add 1 mL. of secondary antibody and incubate for 2 hours at RT on rotator. -PBS + 1 % NDS + 0.3 % triton X 100 + anti-rabbit Cy3 (Jackson Immunoresearch Labs) at 1:60011) Perform 3 X 10 minute PBS washes (1 mL volume) on rotator12) Mount tissue sections on gelatin coated slides and allow tissue to dry13) Run slides through alcohol gradients (70 %, 90 %, 100 %, xylene) leaving slide in each alcohol and xylene for 2 minutes14) Coverslip with DPX mountant (FLUKA) ** Note: 24 well plates may be substituted for microcentrifuge tubes. Use flat top bench rotator instead of upright rotator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TACR1 Antikörper Target Details TACR1 Anwendungsinformationen Referenzen für anti-TACR1 Antikörper (ABIN152451) Bilder zurück nach oben
Format Liquid
Buffer Whole antisera
Buffer contains: No Preservative
Konservierungs-mittel Without preservative
Handhabung Avoid freeze-thaw cycles
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Referenzen für anti-TACR1 Antikörper (ABIN152451)

Produktdetails anti-TACR1 Antikörper Target Details TACR1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Li, Guo, Sun, Li, Ma, Zhang, Guan, Li, Wang: "Botulinum toxin type A and gabapentin attenuate postoperative pain and NK1 receptor internalization in rats." in: Neurochemistry international, Vol. 116, pp. 52-62, 2018

Morinaga, Nakamuta, Yamamoto: "Hypoxia-induced increases in serotonin-immunoreactive nerve fibers in the medulla oblongata of the rat." in: Acta histochemica, Vol. 118, Issue 8, pp. 806-817, 2016 (Probematerial (Species): Human).

Wu, Zhang, Qian, Guo, Yang, Huang, Luo, Xiang, Deng, Dai, Peng, Pan: "Antinociceptive effects of endomorphin-2: suppression of substance P release in the inflammatory pain model rat." in: Neurochemistry international, Vol. 82, pp. 1-9, 2015 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry (Frozen Sections)

Li, Fotinos, Wu, Chen, Zhu, Baranov, Tu, Zhou, Sinha, Kristal, Wang: "N-acetyl-L-tryptophan delays disease onset and extends survival in an amyotrophic lateral sclerosis transgenic mouse model." in: Neurobiology of disease, Vol. 80, pp. 93-103, 2015 (Probematerial (Species): Mouse (Murine)). Weitere Details: Western Blotting

Grip, Lonne-Rahm, Holst, Johansson, Nordlind, Theodorsson, El-Nour: "Substance P alterations in skin and brain of chronically stressed atopic-like mice." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 2, pp. 199-205, 2013 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Ramalho, Almeida, Fernandes, Costa, Pirraco, Guardão, Delgado, Moreira, Soares: "Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype." in: Life sciences, Vol. 93, Issue 4, pp. 169-77, 2013 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Luo, Li, Zhang, Zheng, Wang, Nie, Zhang, Su: "Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 62, Issue 12, pp. 1053-61, 2013 (Probematerial (Species): Guinea Pig). Weitere Details: Western Blotting

Yang, Yan, Zhang, Wang: "Substance P participates in immune-mediated hepatic injury induced by concanavalin A in mice and stimulates cytokine synthesis in Kupffer cells." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 459-464, 2013 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence

Lin, Moore, Jones, McGlashon, Talman: "Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 47, pp. 18608-17, 2013 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Talman, Dragon, Jones, Moore, Lin: "Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat." in: Cellular and molecular neurobiology, Vol. 32, Issue 7, pp. 1119-26, 2012 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Caioli, Curcio, Pieri, Antonini, Marolda, Severini, Zona: "Substance P receptor activation induces downregulation of the AMPA receptor functionality in cortical neurons from a genetic model of Amyotrophic Lateral Sclerosis." in: Neurobiology of disease, Vol. 44, Issue 1, pp. 92-101, 2011 (Probematerial (Species): Mouse (Murine)). Weitere Details: Immunocytochemistry,Immunofluorescence

Commons: "Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3)." in: Journal of chemical neuroanatomy, Vol. 38, Issue 4, pp. 273-81, 2009 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Amadoro, Pieri, Ciotti, Carunchio, Canu, Calissano, Zona, Severini: "Substance P provides neuroprotection in cerebellar granule cells through Akt and MAPK/Erk activation: evidence for the involvement of the delayed rectifier potassium current." in: Neuropharmacology, Vol. 52, Issue 6, pp. 1366-77, 2007 (Probematerial (Species): Rat (Rattus)).

Broccardo, Linari, Agostini, Amadoro, Carpino, Ciotti, Petrella, Petrozza, Severini, Improta: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Broccardo, Ciotti, Linari, Agostini, Petrella, Amadoro, Severini, Improta: "Immunocytochemical distribution of NK-1 and NK-3 tachykinin receptors in isolated pancreatic acini of guinea pigs and rats." in: Peptides, Vol. 26, Issue 11, pp. 2351-4, 2005

Commons, Connolley, Valentino: "A neurochemically distinct dorsal raphe-limbic circuit with a potential role in affective disorders." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 28, Issue 2, pp. 206-15, 2003 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunocytochemistry,Immunofluorescence

Mounir, Parent: "The expression of neurokinin-1 receptor at striatal and pallidal levels in normal human brain." in: Neuroscience research, Vol. 44, Issue 1, pp. 71-81, 2002 (Probematerial (Species): Human). Weitere Details: Western Blotting,Immunohistochemistry

Ma, Huang, Chien, Wu, Chen: "Temporal decrease in renal sensory responses in rats after chronic ligation of the bile duct." in: American journal of physiology. Renal physiology, Vol. 283, Issue 1, pp. F164-72, 2002 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Kc, Haxhiu, Tolentino-Silva, Wu, Trouth, Mack: "Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites." in: Respiratory physiology & neurobiology, Vol. 133, Issue 1-2, pp. 75-88, 2002

Yip, Chahl: "Localization of NK1 and NK3 receptors in guinea-pig brain." in: Regulatory peptides, Vol. 98, Issue 1-2, pp. 55-62, 2001 (Probematerial (Species): Guinea Pig). Weitere Details: Immunohistochemistry

Bilder

Produktdetails anti-TACR1 Antikörper Target Details TACR1 Anwendungsinformationen Handhabung Referenzen für anti-TACR1 Antikörper (ABIN152451) zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (ABIN152451) in ...
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN268373] -...
Immunofluorescence (IF) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN268373] -...
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (C-Term) antibody (ABIN152451) Immunohistochemistry: Neurokinin 1 Receptor Antibody [ABIN268373] - Immunohistochemic...