Human Chemokine (C-C Motif) Ligand 13 ELISA Kit

Recommended Chemokine (C-C Motif) Ligand 13 Product (geliefert von: Anmelden zum Anzeigen )

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Chemokine (C-C Motif) Ligand 13 (CCL13) ELISA Kits
  • CKb10
  • MCP-4
  • NCC-1
  • NCC1
  • SCYA13
  • SCYL1
  • MMCP-4
  • MMCP-4A
  • MMCP-4B
  • Mcp-4
  • Mcp4
  • C-C motif chemokine ligand 13
  • chemokine (C-C motif) ligand 13
  • mast cell protease 4
  • CCL13
  • Mcpt4
Epitop
AA 24-98
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
17
5
4
4
3
2
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
7.8-500 pg/mL
Untere Nachweisgrenze
7.8 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Bilder

Produktnummer ABIN1706066
$ 369.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.452625 ABIN625042 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 3-200 pg/mL 3 pg/mL Anmelden zum Anzeigen 4
10.452625 ABIN3198508 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Anmelden zum Anzeigen
10.452625 ABIN1116023 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
10.452625 ABIN2708092 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 7.8-500 pg/mL 7.8 pg/mL Anmelden zum Anzeigen
1 ABIN2851352 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN812079 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 31.2-2000 pg/mL 31.2 pg/mL Anmelden zum Anzeigen
1 ABIN3032959 Competition ELISA Cell Culture Supernatant, Plasma, Serum 3.13-200 pg/mL 3.13 pg/mL Anmelden zum Anzeigen
1 ABIN770959 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Anmelden zum Anzeigen
1 ABIN5652381 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 3.1 pg/mL - 200 pg/mL 3.1 pg/mL Anmelden zum Anzeigen
1 ABIN5525061 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2683646 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Anmelden zum Anzeigen 1
1 ABIN2683645 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 7.8-500 pg/mL 7.8 pg/mL Anmelden zum Anzeigen
1 ABIN4971126 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN2116216 0.78-50 pg/mL 0.78 pg/mL Anmelden zum Anzeigen
1 ABIN2949559 15.625-1000 pg/mL 15.625 pg/mL Anmelden zum Anzeigen
1 ABIN455899 Anmelden zum Anzeigen
1 ABIN5073605 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen

General

Antigen Chemokine (C-C Motif) Ligand 13 (CCL13) ELISA Kits
Epitop AA 24-98
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(17), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 7.8-500 pg/mL
Untere Nachweisgrenze 7.8 pg/mL
Applikation ELISA
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL13/MCP4
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: E.coli
Immunogen sequence: Q24-T98
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: Q24-T98

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung CCL13 (CCL13 ELISA Kit Abstract)
Hintergrund

Protein Function: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Background: Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Synonyms: C-C motif chemokine 13,CK-beta-10,Monocyte chemoattractant protein 4,Monocyte chemotactic protein 4,MCP-4,NCC-1,Small-inducible cytokine A13,C-C motif chemokine 13, long chain,C-C motif chemokine 13, medium chain,C-C motif chemokine 13, short chain,CCL13,MCP4, NCC1, SCYA13,

Full Gene Name: C-C motif chemokine 13

Cellular Localisation: Secreted.
Gen-ID 6357
UniProt Q99616
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Tissue Specificity: Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

Plattentyp Pre-coated
Protokoll human CCL13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL13 has been precoated onto 96-well plates. Standards(E.coli, Q24-T98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL13 amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CCL13 standard solution and each sample is measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 50, Standard deviation: 2.15, CV(%): 4.3
  • Sample 2: n=16, Mean(pg/ml): 191, Standard deviation: 8.98, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 325, Standard deviation: 20.15, CV(%): 6.2,
  • Sample 1: n=24, Mean(pg/ml): 54, Standard deviation: 3.13, CV(%): 5.8
  • Sample 2: n=24, Mean(pg/ml): 223, Standard deviation: 14.05, CV(%): 6.3
  • Sample 3: n=24, Mean(pg/ml): 356, Standard deviation: 27.77, CV(%): 7.8
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

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Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen

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Allgemeine Veröffentlichungen

Lamkhioued, Garcia-Zepeda, Abi-Younes, Nakamura, Jedrzkiewicz, Wagner, Renzi, Allakhverdi, Lilly, Hamid, Luster: "Monocyte chemoattractant protein (MCP)-4 expression in the airways of patients with asthma. Induction in epithelial cells and mononuclear cells by proinflammatory cytokines." in: American journal of respiratory and critical care medicine, Vol. 162, Issue 2 Pt 1, pp. 723-32, 2000

Blanpain, Migeotte, Lee, Vakili, Doranz, Govaerts, Vassart, Doms, Parmentier: "CCR5 binds multiple CC-chemokines: MCP-3 acts as a natural antagonist." in: Blood, Vol. 94, Issue 6, pp. 1899-905, 1999

Naruse, Ueno, Satoh, Nomiyama, Tei, Takeda, Ledbetter, Coillie, Opdenakker, Gunge, Sakaki, Iio, Miura: "A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2." in: Genomics, Vol. 34, Issue 2, pp. 236-40, 1997

Produkt verwendet in:

Garcia-Zepeda, Combadiere, Rothenberg, Sarafi, Lavigne, Hamid, Murphy, Luster et al.: "Human monocyte chemoattractant protein (MCP)-4 is a novel CC chemokine with activities on monocytes, eosinophils, and basophils induced in allergic and nonallergic inflammation that signals through ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5613-26, 1997

Bilder

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Bilder des Herstellers
ELISA image for Chemokine (C-C Motif) Ligand 13 (CCL13) ELISA Kit (ABIN1706066) Human CCL13/MCP4 PicoKine ELISA Kit standard curve