anti-Human TAP2 Antikörper für Immunoprecipitation

Recommended TAP2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) Antikörper
  • ABC18
  • ABCB3
  • APT2
  • D6S217E
  • PSF-2
  • PSF2
  • RING11
  • AI462429
  • Abcb3
  • Ham-2
  • Ham2
  • MTP2
  • Tap-2
  • Y1
  • jas
  • Cim
  • transporter 2, ATP binding cassette subfamily B member
  • transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
  • TAP2
  • Tap2
Epitop
AA 434-703
20
6
3
2
2
1
1
1
1
1
1
1
Reaktivität
Human
87
42
41
Wirt
Maus
84
2
2
Klonalität
Monoklonal
Konjugat
Dieser TAP2 Antikörper ist unkonjugiert
5
5
5
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
39
35
26
26
17
14
6
4
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2853577
$ 404.80
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1818320 IHC (p) IP WB Mouse IgG1, kappa AA 434-703 Anmelden zum Anzeigen TAP2-17 0
1 ABIN4357820 IHC IHC (p) IP WB Mouse IgG1 kappa Anmelden zum Anzeigen TAP2-17 0

General

Antigen Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) Antikörper
Epitop AA 434-703
(20), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(87), (42), (41)
Wirt Maus
(84), (2), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TAP2 Antikörper ist unkonjugiert
(5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(39), (35), (26), (26), (17), (14), (6), (4), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TAP2 Antikörper

Target Details TAP2 Anwendungsinformationen Handhabung Bilder
Spezifität This antibody reacts with TAP2 (70 kDa) on Western blotting.
Kreuzreaktivität (Details) Species reactivity (tested):Human.
Produktmerkmale Synonyms: PSF2, RING11, Y1, Antigen peptide transporter 2, ATP-binding cassette sub-family B member3, Peptide supply factor 2, Peptide transporter PSF2, Peptide transporter involved inantigen processing 2
Reinigung Protein-A Agarose Chromatography of hybridoma supernatant.
Immunogen Recombinant Human GST-TAP2 (aa 434-703). Remarks: Hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653with Balb/c mouse splenocyte6
Klon TAP1-28
Isotyp IgG1

Target Details TAP2

Produktdetails anti-TAP2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung ABCB3 / APT2 / TAP2 (TAP2 Antibody Abstract)
Hintergrund The transporter associated with antigen processing (TAP) is a critical component of the antigen presentation pathway. Assembly of peptides with newly synthesized class I molecules is assisted by many proteins. These include calreticulin, ERp57 and tapasin. These components together with TAP and the MHC class I subunits, constitute a large complex called the "MHC class I peptide-loading complex". Upon binding to peptides, class I molecules are released from the assembly complex and transit to the cell surface for immune surveillance by T cells. Two structurally related subunits of the TAP transporter, TAP1 and TAP2, form a complex on the ER membrane that is necessary and sufficient for peptide translocation from the cytosol into the ER. TAP1-mediated ATP hydrolysis is not essential for peptide translocation but that TAP2-mediated ATP hydrolysis is critical, not only for translocation, but for peptide binding.Synonyms: ATP-binding cassette sub-family B member 3, Antigen peptide transporter 2, PSF2, Peptide supply factor 2, Peptide transporter PSF2, Peptide transporter involved in antigen processing 2, RING11, Y1
Gen-ID 6891
UniProt Q03519
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

Anwendungsinformationen

Produktdetails anti-TAP2 Antikörper Target Details TAP2 Handhabung Bilder zurück nach oben
Applikationshinweise Western blotting: 5 μg/mL for chemiluminescence detection system. Positive Controls: Jurkat, Raji, HeLa cells. Immunoprecipitation: 2 μg/200 μL of cell extract from 5x10^6 cells. Flow Cytometry. Immunohistochemistry on Paraffin Embedded Sections: 10 μg mL(Heat treatment is necessary). Microwave oven: 2 times for 10 minutes each in citrate buffer ( pH 6.5)Positive Control: Human Breast Cancer. Detailed procedure is provided in Protocols.
Protokoll SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli's sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. 12) Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 13) Expose to an X-ray film in a dark room for 3 minutes. 14) Develop the film as usual. The condition for exposure and development may vary. Positive Control: Jurkat, Raji, HeLaImmunoprecipitation1) Wash the cells 3 x with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add primary antibody as suggested in the APPLICATIONS into 200 µL of the supernatant.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TAP2 Antikörper Target Details TAP2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives.
Konservierungsmittel Without preservative
Lagerung -20 °C
Informationen zur Lagerung Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months

Bilder

Produktdetails anti-TAP2 Antikörper Target Details TAP2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) (AA 434-703) antibody (ABIN2853577) anti-Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) (AA 434-703) antibody
Western Blotting (WB) image for anti-Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) (AA 434-703) antibody (ABIN2853577) anti-Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) (AA 434-703) antibody (Image 2)
Western Blotting (WB) image for anti-Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) (AA 434-703) antibody (ABIN2853577) anti-Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2) (AA 434-703) antibody (Image 3)