anti-Human MTOR Antikörper für Immunofluorescence

Recommended MTOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
C-Term
68
31
29
22
21
18
15
15
15
11
7
7
6
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
408
221
212
16
8
6
6
6
6
5
4
3
2
1
1
1
Wirt
Kaninchen
356
49
3
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
10
10
9
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
294
156
128
68
59
52
33
19
16
8
8
7
3
3
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN2855088
$ 466.18
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
22 ABIN6263424 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 7
21.57959 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Anmelden zum Anzeigen Polyclonal 0
21.57959 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Anmelden zum Anzeigen Polyclonal 0
17.319916 ABIN5966799 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
17.319916 ABIN5966801 IF IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
15.579589 ABIN3183004 ELISA IF IHC Rabbit IgG pSer2481 Anmelden zum Anzeigen Polyclonal 0
15.579589 ABIN3020376 IF IHC WB Rabbit pSer2448 Anmelden zum Anzeigen Polyclonal 0
14.5 ABIN390217 IF IHC (p) WB Rabbit Ig Fraction AA 2459-2488, Ser2481 Anmelden zum Anzeigen Polyclonal 3
14.319915 ABIN3031742 IF IHC ELISA WB Rabbit Ig Fraction AA 2459-2488 Anmelden zum Anzeigen Polyclonal 0
12.579589 ABIN5618282 ELISA IF IHC Rabbit IgG pSer2481 Anmelden zum Anzeigen Polyclonal 0
12.579589 ABIN5620251 ICC IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
12.579589 ABIN2140768 IF IHC WB Rabbit pSer2448 Anmelden zum Anzeigen Polyclonal 0
12.579589 ABIN2147023 IF IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
12.579589 ABIN6144172 IF IHC IP WB Rabbit Anmelden zum Anzeigen Polyclonal 0
12.579589 ABIN6134672 IF IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 0
11.5 ABIN6256211 ELISA ICC IF IHC WB Rabbit IgG pSer2481 Anmelden zum Anzeigen Polyclonal 1
11.319915 ABIN2705013 IC IF WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
11.319915 ABIN1531447 IF IHC ELISA Rabbit IgG AA 2447-2496, pSer2481 Anmelden zum Anzeigen Polyclonal 0
11.319915 ABIN2487252 ICC IF IP Rabbit IgG Exon 5, Exon 6 Anmelden zum Anzeigen Polyclonal 0
10 ABIN196962 IF IHC (p) WB Rabbit pSer2448 Anmelden zum Anzeigen Polyclonal 4

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Epitop C-Term
(68), (31), (29), (22), (21), (18), (15), (15), (15), (11), (7), (7), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(408), (221), (212), (16), (8), (6), (6), (6), (6), (5), (4), (3), (2), (1), (1), (1)
Wirt Kaninchen
(356), (49), (3)
Klonalität
Polyklonal
Konjugat Dieser MTOR Antikörper ist unkonjugiert
(10), (10), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(294), (156), (128), (68), (59), (52), (33), (19), (16), (8), (8), (7), (3), (3), (2), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MTOR Antikörper

Target Details MTOR Anwendungsinformationen Handhabung Bilder
Produktmerkmale Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
Isotyp IgG
Plasmids, Primers & others

Target Details MTOR

Produktdetails anti-MTOR Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung mTOR (FRAP1 Antibody Abstract)
Hintergrund The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side
Molekulargewicht 289 kDa
Gen-ID 2475
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-MTOR Antikörper Target Details MTOR Handhabung Bilder zurück nach oben
Applikations-hinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Kommentare

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Konservierungs-mittel ProClin
Vorsichtsmaßnahmen This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) ICC/IF Image mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunoflu...
Immunoprecipitation (IP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IP Image Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse ...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immun...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue ex...
Immunoprecipitation (IP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IP Image mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) ChIP Image Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of ei...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Various whole c...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (C-Term) antibody (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse tes...