anti-Pferd Adducin 2 (Beta) Antikörper für Western Blotting
Recommended Adducin 2 (Beta) Antibody (geliefert von: Anmelden zum Anzeigen )
- Antigen
-
Adducin 2 (Beta) (ADD2) Antikörper
Synonyme für dieses Antigen anzeigen- si:dkey-202n14.5
- ADDB
- 2900072M03Rik
- add97
- beta-ADD
- adducin 2
- adducin 2 (beta)
- ADD2
- add2
- Add2
- Epitop
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Middle Region
Alternativen - Reaktivität
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Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
Alternativen - Wirt
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Kaninchen
Alternativen - Klonalität
- Konjugat
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Unkonjugiert
Alternativen - Applikation
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Immunofluorescence (IF), Western Blotting (WB)
Alternativen - Optionen
- Hersteller
- Anmelden zum Anzeigen
- Hersteller Produkt- Nr.
- Anmelden zum Anzeigen
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Bilder
Produktnummer ABIN2784900
$ 289.00
Zzgl. Versandkosten $45.00
Zzgl. Versandkosten $45.00
| Relevance Score | ABIN | Application | Konjugat | Host | Isotype | Epitope | Hersteller | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN463758 | WB | Rabbit | IgG | AA 648-697 | Anmelden zum Anzeigen | Polyclonal | 0 | ||
| 1 | ABIN1891523 | IP WB | Rabbit | IgG | AA 1-50 | Anmelden zum Anzeigen | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Adducin 2 (Beta) (ADD2) Antikörper |
| Epitop | Middle Region Alternativen |
| Reaktivität | Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus) Alternativen |
| Wirt | Kaninchen Alternativen |
| Klonalität | |
| Konjugat | Unkonjugiert Alternativen |
| Applikation |
Immunofluorescence (IF), Western Blotting (WB)
Alternativen
|
|
1 Publikation vorhanden |
| Hersteller | Anmelden zum Anzeigen |
ProduktdetailsAntigendetails Anwendungsinformationen Handhabung Referenzen Bilder |
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| Blocking Peptide | Zu diesem Produkt passt das Blocking Peptide ABIN972930. |
| Sequenz | VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS |
| Homologie | Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 100% |
| Produktmerkmale | This is a rabbit polyclonal antibody against ADD2. It was validated on Western Blot using a cell lysate as a positive control. |
| Reinigung | Affinity Purified |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human ADD2 |
AntigendetailsProduktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben |
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| Antigen | |
| Andere Bezeichnung | ADD2 (ADD2 Antibody Abstract) |
| Hintergrund |
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known. Alias Symbols: ADDB Protein Interaction Partner: UBC, APP, RPH3A, PRKACA, PRKCD, PTPRZ1, FYN, CALM1, ADD2, ADD1, Protein Size: 726 |
| Molekulargewicht | 80 kDa |
| Gen-ID | 119 |
| NCBI Accession | NM_001617, NP_001608 |
| UniProt | P35612 |
| Pathways | Regulation of Actin Filament Polymerization |
AnwendungsinformationenProduktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben |
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| Applikationshinweise | Optimal working dilutions should be determined experimentally by the investigator. |
| Kommentare |
Antigen size: 726 AA |
| Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben |
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| Format | Liquid |
| Konzentration | Lot specific |
| Buffer | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose. |
| Konservierungsmittel | Sodium azide |
| Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handhabung | Avoid repeated freeze-thaw cycles. |
| Lagerung | -20 °C |
| Informationen zur Lagerung | For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
ReferenzenProduktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben |
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| Allgemeine Veröffentlichungen |
Kato, Miyata, Tabara, Katsuya, Yanai, Hanada, Kamide, Nakura, Kohara, Takeuchi, Mano, Yasunami, Kimura, Kita, Ueshima, Nakayama, Soma, Hata, Fujioka, Kawano, Nakao, Sekine, Yoshida, Nakamura, Saruta, Ogihara, Sugano, Miki, Tomoike: "High-density association study and nomination of susceptibility genes for hypertension in the Japanese National Project." in: Human molecular genetics, Vol. 17, Issue 4, pp. 617-27, 2008 |
BilderProduktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben |
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| Bilder des Herstellers |
WB Suggested Anti-ADD2 Antibody Titration: 0.2-1 ug/mlELISA Titer: ... 
anti-Adducin 2 (Beta) (ADD2) (Middle Region) antibody (Image 2)
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