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VEGF Protein

VEGF Spezies: Maus Wirt: Yeast (Pichia pastoris) > 97 % by SDS-PAGE. Active
Produktnummer ABIN988283
  • Target Alle VEGF Proteine anzeigen
    VEGF (Vascular Endothelial Growth Factor (VEGF))
    Biologische Aktivität
    Active
    Spezies
    • 12
    • 6
    • 1
    • 1
    Maus
    Quelle
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Yeast (Pichia pastoris)
    Produktmerkmale
    Resuspend cells in assay media (Earle Salts 199X Gibco #11150, without h-EGF) containing 10 % HI-FBS and transfer 100 ul/well to assay plate (5,100 cells/well passage#2). Serial dilute murine VEGF in assay media containing 10 % HI-FBS and transfer 100ml/well to cells in assay plate. Final assay volume is 200 ml/well, containing 10 % HI-FBS, and murine VEGF as indicated 89 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well, Incubate 37°C and read at OD 490 nm. The ED50 for the effect is typically 4.1 - 6.2 ng/ml.
    Reinheit
    > 97 % by SDS-PAGE.
    Endotoxin-Niveau
    level is less than 0.1 ng per myg (1 EU/myg) of VEGF
    Top Product
    Discover our top product VEGF Protein
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Lagerung
    -20 °C
  • Target
    VEGF (Vascular Endothelial Growth Factor (VEGF))
    Andere Bezeichnung
    Vascular Endothelial Growth Factor (VEGF Produkte)
    Synonyme
    MVCD1 Protein, VEGF Protein, VPF Protein, Vegf Protein, Vegf120 Protein, Vegf164 Protein, Vegf188 Protein, Vpf Protein, VEGF-A Protein, VEGF164 Protein, eVEGF120 Protein, eVEGF164 Protein, vegf-a Protein, vegf Protein, vascular endothelial growth factor A Protein, vascular endothelial growth factor A L homeolog Protein, vascular endothelial growth factor Protein, vascular endothelial growth factor precursor Protein, VEGFA Protein, Vegfa Protein, vegfa.L Protein, VEGF Protein, vegf Protein
    Hintergrund
    VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Synonym: VEGF 6. Formulation: Lyophilized from 25 mM HEPES and 150 mM NaCl, pH 7.0.
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