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Protein G Protein (HRP)

Spezies: Streptococcus Native ELISA, WB
Produktnummer ABIN964530
  • Target Alle Protein G Proteine anzeigen
    Protein G
    Protein-Typ
    Native
    Spezies
    Streptococcus
    Wirt
    Bitte anfragen
    Aufreinigungstag / Konjugat
    Dieses Protein G Protein ist gelabelt mit HRP.
    Applikation
    ELISA, Western Blotting (WB)
    Spezifität
    Protein G Peroxidase was prepared from chromatographically pure Protein G. Protein G Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein G. No reaction was observed against anti-Protein A.
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    Discover our top product Protein G Protein
  • Applikationshinweise
    Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein GA Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.
    Kommentare

    Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein GA Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target
    Protein G
    Abstract
    Protein G Produkte
    Hintergrund
    Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. Protein G also binds to albumin. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
    Synonyms: HRP, peroxidase, Horseradish peroxidase protein G
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