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AKR1B10 Protein (AA 1-316)

AKR1B10 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Produktnummer ABIN935035
  • Target Alle AKR1B10 Proteine anzeigen
    AKR1B10 (Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 1-316
    Spezies
    Human
    Quelle
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
    Produktmerkmale
    Purified recombinant Human AKR1 B10 protein
    Expression System: E.coli
    Bioactivity: Specific activity: approximately 0.32 - 0.4 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 °C. Specific activity was expressed as units/mg protein.
    Molecular weight on SDS-PAGE will appear higher.
    Reinheit
    > 95 % pure
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    Discover our top product AKR1B10 Protein
  • Applikationshinweise
    AKR1B10 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Testdurchführung
    1. Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
      2. Add 50 µL of recombinant AKR1B10 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer .
      3. Mix by inversion and Incubate at 25 °C for
      2.5 minutes.
      4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
      5. Record the increase at A340 nm for 3 minutes.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    RT/-20 °C
    Informationen zur Lagerung
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    AKR1B10 (Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))
    Andere Bezeichnung
    AKR1B10 (AKR1B10 Produkte)
    Synonyme
    AKR1B11 Protein, AKR1B12 Protein, ALDRLn Protein, ARL-1 Protein, ARL1 Protein, HIS Protein, HSI Protein, 2310005E10Rik Protein, Akr1b16 Protein, AKR Protein, AKR1B10 Protein, Akr1b10 Protein, aldo-keto reductase family 1 member B10 Protein, aldo-keto reductase family 1, member B10 (aldose reductase) Protein, aldo-keto reductase family 1 member B10-like 2 Protein, AKR1B10 Protein, Akr1b10 Protein, AKR1B10L2 Protein, LOC100585902 Protein, LOC100723118 Protein, LOC101107697 Protein
    Hintergrund
    AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E. coli and purified by using conventional chromatography.
    Alternative Names: AKRB0 1, AKR1B12 protein, member B10 protein, AKRB0-1 protein, AKR1B10 protein, HIS protein, SI reductase protein, HIS protein, Aldose reductase related protein protein, AKR1B10, AKRB0-1, ARL1 protein, Aldo-keto reductase family 1 protein, Small intestine reductase., member B10 Aldose reductase like protein, ARL-1 protein, ALDRLn protein, AKRB0 1 protein, hARP protein, ARL 1 protein, AKR1B11 protein, Aldo-keto reductase family 1 protein
    Molekulargewicht
    36 kDa (316 AA)
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