Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

AKR1B1 Protein (AA 1-316)

AKR1B1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Produktnummer ABIN935005
  • Target Alle AKR1B1 Proteine anzeigen
    AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 1-316
    Spezies
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Quelle
    • 5
    • 4
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Applikation
    SDS-PAGE (SDS)
    Sequenz
    MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
    Produktmerkmale
    Purified recombinant Human Aldose reductase protein
    Expression System: E.coli
    Bioactivity: Specific activity: approximately 0.5-0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH/min at 25 °C.
    Molecular weight on SDS-PAGE will appear higher.
    Reinheit
    > 95 % pure
    Endotoxin-Niveau
    < 1.0 EU per µg of protein (determined by LAL method)
    Top Product
    Discover our top product AKR1B1 Protein
  • Applikationshinweise
    Aldose reductase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Testdurchführung
    1. Prepare a 1 mL reaction mix into a suitable container : The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
      2. Add 50 µL of recombinant AKR1B1 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer.
      3. Mix by inversion and Incubate at 25 °C for
      2.5 minutes.
      4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
      5. Record the increase at A340 nm for 3 minutes.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.
    Konservierungsmittel
    Dithiothreitol (DTT)
    Vorsichtsmaßnahmen
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    RT/-20 °C
    Informationen zur Lagerung
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
    Andere Bezeichnung
    Aldose reductase (AKR1B1 Produkte)
    Synonyme
    ADR Protein, ALDR1 Protein, ALR2 Protein, AR Protein, ALDRED Protein, ALR-P-I Protein, Akr1b3 Protein, Akr1b4 Protein, Aldr1 Protein, Alr Protein, RATALDRED Protein, akr1b7 Protein, zgc:86611 Protein, aldo-keto reductase family 1 member B Protein, aldo-keto reductase family 1, member B1 (aldose reductase) Protein, aldo-keto reductase family 1, member B1 (aldose reductase) S homeolog Protein, AKR1B1 Protein, Akr1b1 Protein, akr1b1.S Protein, akr1b1 Protein
    Hintergrund
    Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: ALR2 protein, member B1 AKR1B 1 protein, ALDR1 protein, aldehyde reductase 1 protein, aldo-keto reductase family 1 protein, AKR1B1 protein, Aldo-keto reductase family1 member B1 protein, AR protein, aldr-1 protein, Lii5-2 CTCL tumor protein protein, ADR protein, Aldose reductase protein, member B1 aldr 1 protein, Aldo-keto reductase family1 protein, low Km aldose reductase., Aldehyde reductase protein
    Molekulargewicht
    35.8 kDa (316 AA)
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process
Sie sind hier:
Kundenservice