Purified recombinant Human Aldose reductase protein Expression System: E.coli Bioactivity: Specific activity: approximately 0.5-0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH/min at 25 °C. Molecular weight on SDS-PAGE will appear higher.
Reinheit
> 95 % pure
Endotoxin-Niveau
< 1.0 EU per µg of protein (determined by LAL method)
Crystallography grade
AKR1B1
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, SDS, WB, Crys
AKR1B1
Spezies: Human, Maus, Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
WB, PC
Applikationshinweise
Aldose reductase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Testdurchführung
Prepare a 1 mL reaction mix into a suitable container : The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH. 2. Add 50 µL of recombinant AKR1B1 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer. 3. Mix by inversion and Incubate at 25 °C for 2.5 minutes. 4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340 nm for 3 minutes.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.
Konservierungsmittel
Dithiothreitol (DTT)
Vorsichtsmaßnahmen
This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
RT/-20 °C
Informationen zur Lagerung
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Target
AKR1B1
(Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
ADR Protein, ALDR1 Protein, ALR2 Protein, AR Protein, ALDRED Protein, ALR-P-I Protein, Akr1b3 Protein, Akr1b4 Protein, Aldr1 Protein, Alr Protein, RATALDRED Protein, akr1b7 Protein, zgc:86611 Protein, aldo-keto reductase family 1 member B Protein, aldo-keto reductase family 1, member B1 (aldose reductase) Protein, aldo-keto reductase family 1, member B1 (aldose reductase) S homeolog Protein, AKR1B1 Protein, Akr1b1 Protein, akr1b1.S Protein, akr1b1 Protein
Hintergrund
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques. Alternative Names: ALR2 protein, member B1 AKR1B 1 protein, ALDR1 protein, aldehyde reductase 1 protein, aldo-keto reductase family 1 protein, AKR1B1 protein, Aldo-keto reductase family1 member B1 protein, AR protein, aldr-1 protein, Lii5-2 CTCL tumor protein protein, ADR protein, Aldose reductase protein, member B1 aldr 1 protein, Aldo-keto reductase family1 protein, low Km aldose reductase., Aldehyde reductase protein