Telefon:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antikoerper-online.de

Dihydrodiol Dehydrogenase (DDH) (AA 1-323) (Active) protein (His tag) Protein

DDH Spezies: Human Quelle: Escherichia coli (E. coli) Recombinant > 90 %
Produktnummer ABIN805926
$3,055.38
Zzgl. Versandkosten $45.00
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 2 bis 3 Werktagen
  • Target
    Dihydrodiol Dehydrogenase (DDH)
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 1-323
    Spezies
    Human
    Quelle
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    His tag
    Sequenz
    MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
    Produktmerkmale
    Biological Activity: Specific activity: approximately 0.15 - 0.2 units/mg.Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein.
    AKR1C1 Human Recombinant fused to 20 AA His Tag at N-terminal produced in E. coli is a single, non-glycosylated, polypeptide chain containing 343 AA (1-323 AA) and having a molecular mass of 38.9 kDa.
    Aufreinigung
    Purified by proprietary chromatographic techniques
    Reinheit
    > 90 %
    Sterilität
    Sterile filtered
  • Applikationshinweise
    Optimal working dilutions should be determined experimentally by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    The AKR1C1 solution contains 20 mM Tris-HCl pH 8, 0.1 mM DTT and 20 % glycerol.
    Handhabung
    Please avoid freeze thaw cycles.
    Lagerung
    -20 °C
  • Target
    Dihydrodiol Dehydrogenase (DDH)
    Synonyme
    2-ALPHA-HSD, 20-ALPHA-HSD, C9, DD1, DD1/DD2, DDH, DDH1, H-37, HAKRC, HBAB, MBAB, aldo-keto reductase family 1 member C1, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase), AKR1C1
    Hintergrund
    AKR1C1 transfers progesterone to its inactive state or in other words catalyzes the reaction of 20-alpha-hydroxy progesterone (20-alpha-OHP). In the liver and intestine. AKR1C1 transfers bile and monitors the intrahepatic bile acid concentration though it has a low bile-binding ability. AKR1C1 participates in myelin formation. AKR1C1 is part of the aldo/keto reductase superfamily, which has over 40 known enzymes which catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors thus display overlapping but distinct substrate specificity

    Synonyms: DDH1, DDH, HAKRC, 20-alpha-HSD, DD1/DD2, HBAB, C9, DD1, H-37, MBAB, MGC8954, 2-ALPHA-HSD, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, Indanol dehydrogenase, Dihydrodiol dehydrogenase 1/2, Chlordecone reductase homolog HAKRC, High-affinity hepatic bile acid-binding protein
    Molekulargewicht
    22.9 kDa
    Pathways
    Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
Sie sind hier:
help Kundenservice