GADD45A Protein (His tag)

Produktnummer ABIN7668857

Dieses Recombinant GADD45A-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für GADD45A Protein (His tag) (ABIN7668857)

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Protein-Typ: Recombinant

Biologische Aktivität: Inactive

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 %

Produktdetails

Aufreinigungstag / Konjugat: Dieses GADD45A Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human GADD45A protein (His tag)

Sequenz: Met 1-Arg 165

Aufreinigung: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 10 EU/mg of the protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Andere Bezeichnung: GADD45A

Hintergrund: GADD45A is a member of the GADD45 Family, and has been found to associate with several cytoplasmic and nuclear factors and has been implicated in several cellular functions, including MAPK signaling, cell cycle regulation, DNA repair and genomic stability, apoptosis, and immune responses. The GADD45 Family of genes is rapidly induced by different stressors, including differentiation-inducing cytokines, and there is a large body of evidence that their cognate proteins are key players in cellular stress responses. GADD45A protein has been reported to interact with multiple important cellular proteins, including Cdc2 protein kinase, proliferating cell nuclear antigen (PCNA), p21Waf1/Cip1 protein, core histone protein and MTK/MEKK4, an up-stream activator of the JNK/SAPK pathway, indicating that GADD45A may play important roles in the control of cell cycle checkpoint, DNA repair process, and signaling transduction. GADD45A expression in response to genotoxic stress illustrates a more complex scenario, wherein transcriptional changes operate in concert with mRNA turnover and translational regulation. GADD45A was the first stress-inducible gene determined to be up-regulated by p53 and is also a target for the p53 homologues, p63 and p73. The decreased GADD45A expression is also considered a survival mechanism, as cancer cells without this control can evade the apoptotic pathway leading to increased tumourigenesis. As GADD45A is an essential component of many metabolic pathways that control proliferating cancer cells, it presents itself as an emerging drug target worthy of further investigation.,DNA damage-inducible transcript 1 protein,GADD45A,Growth arrest and DNA damage-inducible protein GADD45 alpha

Molekulargewicht:

calculated_mw: 18.0 kDa

observed_mw: 23 kDa

UniProt: P24522

Pathways: p53 Signalweg, Zellzyklus