NR1I2 Protein (AA 1-431) (His tag)

Produktnummer ABIN7562442

Produktdetails

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-431

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses NR1I2 Protein ist gelabelt mit His tag.

Verwendungszweck: Custom-made recombinant Nr1i2 Protein expressed in mammalian cells.

Sequenz: MRPEESWSRV GLVQCEEADS ALEEPINVEE EDGGLQICRV CGDKANGYHF NVMTCEGCKG FFRRAMKRNV RLRCPFRKGT CEITRKTRRQ CQACRLRKCL ESGMKKEMIM SDAAVEQRRA LIKRKKREKI EAPPPGGQGL TEEQQALIQE LMDAQMQTFD TTFSHFKDFR LPAVFHSGCE LPEFLQASLL EDPATWSQIM KDRVPMKISL QLRGEDGSIW NYQPPSKSDG KEIIPLLPHL ADVSTYMFKG VINFAKVISY FRDLPIEDQI SLLKGATFEM CILRFNTMFD TETGTWECGR LAYCFEDPNG GFQKLLLDPL MKFHCMLKKL QLHKEEYVLM QAISLFSPDR PGVVQRSVVD QLQERFALTL KAYIECSRPY PAHRFLFLKI MAVLTELRSI NAQQTQQLLR IQDSHPFATP LMQELFSSTD G Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Andere Bezeichnung: Nr1i2 (NR1I2 Produkte)

Hintergrund: Nuclear receptor subfamily 1 group I member 2 (Orphan nuclear receptor PXR) (Pregnane X receptor),FUNCTION: Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes (By similarity). Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN). {ECO:0000250, ECO:0000269|PubMed:12569201, ECO:0000269|PubMed:19297428}.

Molekulargewicht: 49.6 kDa

UniProt: O54915

AlphaFold: O54915

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway