ATG10 Protein (AA 1-215) (His tag)

Produktnummer ABIN7559677

Dieses Recombinant ATG10-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für ATG10 Protein (AA 1-215) (His tag) (ABIN7559677)

Target: ATG10 (Autophagy Related 10 (ATG10))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-215

Aufreinigungstag / Konjugat: Dieses ATG10 Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Atg10 Protein expressed in mammalian cells.

Sequenz: MEDEFFGEKS FQHYCAEFIR HSQQIGDGWE WRTAKECSDG YMCKTQFRIK NEASTPHVGT PASVLTCLPT EENLELPMDD SEVTRPAAVA EVIKHEYHVL YSCSYQVPVL YFRASFLDGR PLALEDIWEG VHECYKPRLL QGPWDTITQQ EHPILGQPFF VLHPCKTNEF MTAVLKNSQK INRNVNYITS WLSLVGPVVG LNLPLSYAKA TSQSE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: ATG10 (Autophagy Related 10 (ATG10))

Andere Bezeichnung: Atg10

Hintergrund: Ubiquitin-like-conjugating enzyme ATG10 (EC 2.3.2.-) (Autophagy-related protein 10) (APG10-like) (mAPG10),FUNCTION: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis. {ECO:0000269|PubMed:12890687, ECO:0000269|PubMed:20723759, ECO:0000269|PubMed:21367888}.

Molekulargewicht: 24.6 kDa

UniProt: Q8R1P4

AlphaFold: Q8R1P4

Pathways: ER-Nucleus Signaling, Autophagie