RSPO1 Protein (His tag)
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- Target Alle RSPO1 Proteine anzeigen
- RSPO1 (R-Spondin 1 (RSPO1))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- CHO Cells
- Aufreinigungstag / Konjugat
- Dieses RSPO1 Protein ist gelabelt mit His tag.
- Sequenz
- Arg31-Ala263
- Reinheit
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
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R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)
RSPO1 Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)RSPO1 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (AA 21-146) protein (His tag)RSPO1 Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 Spezies: Maus Wirt: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)Crystallography grade RSPO1 Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)Crystallography grade RSPO1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- RSPO1 (R-Spondin 1 (RSPO1))
- Andere Bezeichnung
- R spondin 1 (RSPO1 Produkte)
- Synonyme
- RGD1565558 Protein, CRISTIN3 Protein, RSPO Protein, R-spondin Protein, Rspondin Protein, wu:fj81e05 Protein, zgc:92153 Protein, R-spondin 1 Protein, Rspo1 Protein, RSPO1 Protein, LOC100049655 Protein, rspo1 Protein
- Hintergrund
- R-spondin-1, RSPO1, CRISTIN3, FLJ40906, RP11-566C13.1, RSPO,The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
- Molekulargewicht
- 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
- UniProt
- Q2MKA7
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