Recombinant Mouse PVRIG Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser35-Asp165.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse PVRIG, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse Nectin-2, His Tag with the EC50 of 0.65ug/ml determined by ELISA. See testing image for detail.
Crystallography grade
PVRIG
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
C7orf15 Protein, PVR related immunoglobulin domain containing Protein, PVRIG Protein
Hintergrund
Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.
Molekulargewicht
42.70 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.