Dieses Prolactin Receptor Protein ist gelabelt mit His tag.
Verwendungszweck
Human PRLR Protein
Sequenz
Gln25-Asp234
Produktmerkmale
Recombinant Human PRLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-Asp234.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human PRLR, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 78.7ng/ml determined by ELISA. The affinity constant of 0.96nM as determined in SPR assay (Biacore T200). See testing image for detail.
PRLR
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by SDS-PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150nM Nacl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Prolactin receptor (PRLR) is highly expressed in a subset of human breast cancer and prostate cancer, which makes it a potential target for cancer treatment. In clinical trials, the blockade of PRLR was shown to be safe but with poor efficacy. It is therefore urgent to develop new therapies against PRLR target. Bispecific antibodies (BsAbs) could guide immune cells toward tumor cells, and produced remarkable effects in some cancers.
Molekulargewicht
25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.