MFAP4
(Microfibrillar-Associated Protein 4 (MFAP4))
Protein-Typ
Recombinant
Proteineigenschaft
AA 22-255
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses MFAP4 Protein ist gelabelt mit His-DYKDDDDK Tag.
Verwendungszweck
Human MFAP4 Protein
Sequenz
Val22-Ala255
Produktmerkmale
Recombinant Human MFAP4 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Val22-Ala255.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human MFAP4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MFAP4 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
MFAP4
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
MFAP4
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
> 97 %
SDS, WB, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
MFAP4
(Microfibrillar-Associated Protein 4 (MFAP4))
1110007F23Rik Protein, Magp-36 Protein, zgc:77076 Protein, microfibril-associated glycoprotein 4 Protein, microfibrillar-associated protein 4 Protein, microfibril associated protein 4 S homeolog Protein, microfibril associated protein 4 Protein, CpipJ_CPIJ000434 Protein, CpipJ_CPIJ006120 Protein, CpipJ_CPIJ010089 Protein, CpipJ_CPIJ018551 Protein, CpipJ_CPIJ020296 Protein, mfap4 Protein, mfap4.S Protein, MFAP4 Protein, Mfap4 Protein
Hintergrund
Microfibril-associated glycoprotein 4 (MFAP4) is an extracellular matrix protein belonging to the fibrinogen-related protein superfamily. MFAP4 is produced by vascular smooth muscle cells and is highly enriched in the blood vessels of the heart and lung, where it is thought to contribute to the structure and function of elastic fibers. Genetic studies in humans have implicated MFAP4 in the pathogenesis of Smith-Magenis syndrome, in which patients present with multiple congenital abnormalities and mental retardation, as well as in the severe cardiac malformation left-sided congenital heart disease.
Molekulargewicht
28.6 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.