Recombinant Rhesus macaque Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe31-Pro993(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Rhesus macaque ITGAV&ITGB6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha-V Antibody, hFc Tag with the EC50 of 32.4ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.
Molekulargewicht
112.1 kDa (ITGAV)&79 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.