Dieses ERBB3 Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Human Her3/ErbB3 Protein
Sequenz
Ser20-Thr643
Produktmerkmale
Recombinant Human Her3/ErbB3 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser20-Thr643.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Her3, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. See testing image for detail.
ERBB3
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ERBB3
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 93 % as determined by SDS-PAGE
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).
Molekulargewicht
71.6 kDa. Due to glycosylation, the protein migrates to 72-75 kDa based on Tris-Bis PAGE result.