Selectin E/CD62e Protein (AA 22-556) (His-Avi Tag)

Produktnummer ABIN7274572

Produktdetails

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-556

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Selectin E/CD62e Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human E-Selectin/CD62E Protein

Sequenz: Trp22-Pro556

Produktmerkmale: Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: E-Selectin (SELE Produkte)

Synonyme: SELE Protein, CD62E Protein, ELAM Protein, ELAM1 Protein, ESEL Protein, LECAM2 Protein, E-selectin Protein, Elam Protein, selectin E Protein, selectin, endothelial cell Protein, SELE Protein, Sele Protein

Hintergrund: E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Molekulargewicht: 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling