VTCN1 Protein (AA 29-258) (His-Avi Tag)

Produktnummer ABIN7273952

Produktdetails

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 29-258

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses VTCN1 Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human B7-H4 Protein

Sequenz: Phe29-Ala258

Produktmerkmale: Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe29-Ala258.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-H4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Andere Bezeichnung: B7-H4 (VTCN1 Produkte)

Hintergrund: B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .

Molekulargewicht: 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.