CD166 Protein (AA 28-526) (His tag,Biotin)

Produktnummer ABIN7273816

Produktdetails

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Protein-Typ: Recombinant

Proteineigenschaft: AA 28-526

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD166 Protein ist gelabelt mit His tag,Biotin.

Verwendungszweck: Biotinylated Human ALCAM/CD166 Protein (Primary Amine Labeling)

Sequenz: Trp28-Ala526

Produktmerkmale: Recombinant Biotinylated Human ALCAM/CD166 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Trp28-Ala526.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-ALCAM Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ALCAM, His Tag with the EC50 of 3.7μg/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Andere Bezeichnung: ALCAM (ALCAM Produkte)

Synonyme: CD166 Protein, MEMD Protein, ALCAM Protein, BEN Protein, DM-GRASP Protein, JC7 Protein, SC1 Protein, AI853494 Protein, MuSC Protein, alcam Protein, cd166 Protein, memd Protein, fb53h09 Protein, fb80a10 Protein, nlcam Protein, si:ch211-190h2.4 Protein, wu:fb53h09 Protein, wu:fb80a10 Protein, wu:fc14b01 Protein, activated leukocyte cell adhesion molecule Protein, activated leukocyte cell adhesion molecule a Protein, activated leukocyte cell adhesion molecule L homeolog Protein, CD166 antigen homolog Protein, activated leukocyte cell adhesion molecule b Protein, ALCAM Protein, Alcam Protein, alcama Protein, alcam.L Protein, alcam Protein, cd166 Protein, alcamb Protein

Hintergrund: Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.

Molekulargewicht: 57 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.