CLPS Protein (His tag)

Produktnummer ABIN7321063

Produktdetails

Target: CLPS (Colipase, Pancreatic (CLPS))

Protein-Typ: Recombinant

Spezies: Ratte

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CLPS Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Rat CLPS/Colipase Protein (His Tag)

Sequenz: Met1-Gln112

Produktmerkmale: A DNA sequence encoding the rat Clps (NP_037271.1) (Met1-Gln112) was expressed with a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg protein as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CLPS (Colipase, Pancreatic (CLPS))

Andere Bezeichnung: CLPS/Colipase (CLPS Produkte)

Synonyme: CLPS Protein, 2200003J09Rik Protein, COLQ Protein, colipase Protein, colipase, pancreatic Protein, CLPS Protein, Clps Protein

Hintergrund:

Background: Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.

Synonym: CLPS,Colipase

Molekulargewicht: 11.9 kDa

NCBI Accession: NP_037271

Pathways: Lipid Metabolism