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CSK Protein (GST tag,His tag)

CSK Spezies: Maus Wirt: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
Produktnummer ABIN7320001
  • Target Alle CSK Proteine anzeigen
    CSK (C-Src tyrosine Kinase (CSK))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    • 10
    • 2
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Baculovirus infected Insect Cells
    Aufreinigungstag / Konjugat
    Dieses CSK Protein ist gelabelt mit GST tag,His tag.
    Verwendungszweck
    Recombinant Mouse CSK/C-Src kinase Protein (His & GST Tag)(Active)
    Sequenz
    Met 1-Leu 450
    Produktmerkmale
    A DNA sequence encoding the mouse CSK (P41241?) (Met 1-Leu 450) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
    Reinheit
    > 85 % as determined by SDS-PAGE
    Endotoxin-Niveau
    < 1.0 EU per μg of the protein as determined by the LAL method.
    Biological Activity Comment
    The specific activity was determined to be 70 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
    Top Product
    Discover our top product CSK Protein
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Frozen, Liquid
    Buffer
    Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
  • Target
    CSK (C-Src tyrosine Kinase (CSK))
    Andere Bezeichnung
    CSK/C-Src kinase (CSK Produkte)
    Synonyme
    AW212630 Protein, C-terminal Src kinase Protein, c-src tyrosine kinase Protein, CSK, non-receptor tyrosine kinase Protein, CSK Protein, Csk Protein
    Hintergrund

    Background: The tyrosine kinase c-Src has been implicated as a modulator of cell proliferation, spreading, and migration. These functions are also regulated by Met. The structure of a large fragment of the c-Src kinase comprises the regulatory and kinase domains and the carboxy-terminal tall. c-Src kinase interactions among domains and is stabilized by binding of the phosphorylated tail to the SH2 domain. This molecule is locked in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase. The protein-tyrosine kinase activity of c-Src kinase is inhibited by phosphorylation of tyr527, within the c-Src c-terminal tail. Genetic and biochemical data have suggested that this negative regulation requires an intact Src homology 2 (SH2) domain. Since SH2 domains recognize phosphotyrosine, it is possible that these two non-catalytic domains associate, and thereby repress c-Src kinase activity. Experiments have suggested that c-Src kinase plays a role in the biological behaviour of colonic carcinoma cells induced by migratory factors such as EGF, perhaps acting in conjunction with FAK to regulate focal adhesion turnover and tumour cell motility. Furthermore, although c-Src kinase has been implicated in colonic tumour progression, in the adenoma to carcinoma in vitro model c-Src is not the driving force for this progression but co-operates with other molecules in carcinoma development. References

    Synonym: AW212630,p50CSK

    Molekulargewicht
    78.5 kDa
    Pathways
    T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events
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