eEF1A1 Protein (full length) (rho-1D4 tag)

Produktnummer ABIN7126039

Produktdetails

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Protein-Typ: Recombinant

Proteineigenschaft: full length

Spezies: CHO cells

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses eEF1A1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Functional Studies (Func), Crystallization (Crys)

Sequenz: MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GSASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK VTKSAQKAQK AK
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor Tu) (EF-Tu) (Eukaryotic elongation factor 1 A-1) (eEF1A-1) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Güteklasse: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Andere Bezeichnung: Elongation factor 1-alpha 1 (EF-1-alpha-1) (EEF1A1 Produkte)

Synonyme: CCS-3 Protein, CCS3 Protein, EE1A1 Protein, EEF-1 Protein, EEF1A Protein, EF-Tu Protein, EF1A Protein, GRAF-1EF Protein, HNGC:16303 Protein, LENG7 Protein, PTI1 Protein, eEF1A-1 Protein, Eef1a2 Protein, Eef1a2l1 Protein, SI Protein, EEF1A2 Protein, EF1A1 Protein, eef1a1 Protein, wu:fj34g08 Protein, zgc:110335 Protein, EEF1A1 Protein, RABEFLA2 Protein, EFL1-alpha Protein, chunp6927 Protein, eef1a Protein, ef1a Protein, ik:tdsubc_2a3 Protein, ik:tdsubc_2b3 Protein, tdsubc_2a3 Protein, wu:fa91c07 Protein, wu:fa94b03 Protein, wu:fi13b09 Protein, xx:tdsubc_2a3 Protein, xx:tdsubc_2b3 Protein, EF-1A Protein, EF-1-ALPHA-S Protein, eef1a-s Protein, eef1as Protein, fj64c02 Protein, wu:fj64c02 Protein, zgc:73138 Protein, eukaryotic translation elongation factor 1 alpha 1 Protein, eukaryotic translation elongation factor 1 alpha 1b Protein, eukaryotic translation elongation factor 1 alpha 1, like 1 Protein, eukaryotic translation elongation factor 1 alpha 1 L homeolog Protein, elongation factor 1-alpha Protein, eukaryotic translation elongation factor 1 alpha 1a Protein, EEF1A1 Protein, Eef1a1 Protein, eef1a1b Protein, eef1a1l1 Protein, eef1a1.L Protein, tufA Protein, eef1a1a Protein

Hintergrund: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. {ECO:0000250|UniProtKB:P68104}.

UniProt: P62629