SARS-CoV-2 Spike Protein (B.1.1.7 - alpha) (His tag)

Produktnummer ABIN6973222

Produktdetails

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: B.1.1.7 - alpha

Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His tag.

Sequenz: AA 16-1213

Spezifität: SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (MALS verified)

Produktmerkmale: SARS-CoV-2 S protein, His Tag is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. HV69-70del / Y144del / N501Y / A570D / D614G / P681H / T716I / S982A / D1118H mutations were identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: MALS verified

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS

Lagerung: -20 °C

Referenzen

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Antigendetails

Target: SARS-CoV-2 Spike

Andere Bezeichnung: SARS-CoV-2 S protein (SARS-CoV-2 Spike Produkte)

Synonyme: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 137.8 kDa