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CD19 Protein (AA 20-291) (His tag,AVI tag,Biotin)

CD19 Spezies: Human Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Produktnummer ABIN6972966
  • Target Alle CD19 Proteine anzeigen
    CD19 (CD19 Molecule (CD19))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 20-291
    Spezies
    • 30
    • 6
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Human
    Quelle
    • 25
    • 6
    • 5
    • 3
    • 3
    • 1
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses CD19 Protein ist gelabelt mit His tag,AVI tag,Biotin.
    Spezifität
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Produktmerkmale
    Biotinylated Human CD19 (20-291) Protein, His,Avitag™ (SPR verified)
    Reinheit
    >90 % as determined by SDS-PAGE.
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product CD19 Protein
  • Applikationshinweise
    SPR verified
    Kommentare

    Ready-to-use Avitag™ biotinylated protein:
    The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Lagerung
    -20 °C
  • Target
    CD19 (CD19 Molecule (CD19))
    Andere Bezeichnung
    CD19 (CD19 Produkte)
    Synonyme
    B4 Protein, CVID3 Protein, AW495831 Protein, CD19 Protein, CD19 molecule Protein, CD19 antigen Protein, CD19 Protein, Cd19 Protein
    Hintergrund
    B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.
    Molekulargewicht
    33.7 kDa
    NCBI Accession
    NP_001761
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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