SARS-CoV-2 Spike Protein (B.1.351 - beta) (rho-1D4 tag)

Produktnummer ABIN6963740

Produktdetails

Target: SARS-CoV-2 Spike

Protein-Typ: Recombinant

Proteineigenschaft: B.1.351 - beta

Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Ligand Binding Assay (LBA)

Verwendungszweck: This is the spike protein of the mutant strain B.1.351, also commonly known as the "South Africa mutant".

Spezifität: Mutation that differ from canonical sequence of SPIKE protein: del 144, K417N, E484K, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H

Produktmerkmale: "SARS CoV-2 full-length Spike B.1.351 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent.

Aufreinigung: affinity chromatography

Reinheit: > 98 % as determined by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses

Kommentare:

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Avoid freeze-thaw cycles

Referenzen

Erickson, Logsdon, Rhea, Hansen, Holden, Banks, Smith, German, Farr, Morley, Weaver, Hirsch, Kovac, Kontsekova, Baumann, Omer, Raber: "Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." in: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).

Bhalla, Payam, Morelli, Sharma, Johnson, Thomson, Jolly, Canfarotta: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).

Arunachalam, Feng, Ashraf, Hu, Walls, Edara, Zarnitsyna, Aye, Golden, Miranda, Green, Threeton, Maness, Beddingfield, Bohm, Scheuermann, Goff, Dufour, Russell-Lodrigue, Kepl, Fiala, Wrenn et al.: "Durable protection against the SARS-CoV-2 Omicron variant is induced by an adjuvanted subunit vaccine. ..." in: Science translational medicine, Vol. 14, Issue 658, pp. eabq4130, (2022) (PubMed).

Funari, Bhalla, Gentile: "Measuring the Radius of Gyration and Intrinsic Flexibility of Viral Proteins in Buffer Solution Using Small-Angle X-ray Scattering." in: ACS measurement science au, Vol. 2, Issue 6, pp. 547-552, (2022) (PubMed).

Antigendetails

Target: SARS-CoV-2 Spike

Andere Bezeichnung: SARS2 Spike glycoprotein (SARS-CoV-2 Spike Produkte)

Synonyme: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Molekulargewicht: 142114 Da

UniProt: P0DTC2