Recombinant Human ACE2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln18-Ser740) of human ACE2 (Accession #Q9BYF1) fused with a Fc tag at the C-terminus.
Reinheit
> 95 % by SDS-PAGE.
Sterilität
0.22 μm filtered
Endotoxin-Niveau
< 0.1 EU/μg of the protein by LAL method.
Biological Activity Comment
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike RBD at 1 μg/mL (100 μL/well) can bind Human ACE2 with a linear range of 0.01-2.4 ng/mL.
ACE2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
ACE2
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Buffer
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.|or This product is stable at ≤ -70°C for up to 1 year from the date of receipt.|For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.