SARS-CoV-2 NSP16, NSP10 protein (His tag,Twin-Strep tag)

Produktnummer ABIN6952709

Produktdetails

Target: SARS-CoV-2 NSP16, NSP10

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag,Twin-Strep tag

Verwendungszweck: SARS-CoV-2 (COVID-19) NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag

Sequenz: AA 1-298

Produktmerkmale: SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag (NS0-C51W3) is expressed from E.coli cells. It contains AA Ser 1 - Asn 298 (NSP16) & Ala 1 - Gln 139 (NSP10) (Accession # YP_009725311.1 (NSP16) & YP_009725306.1 (NSP10). Predicted N-terminus: Met (NSP16) & Met (NSP10)
SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag is produced by co-expression of NSP16 and NSP10, has a calculated MW of 35.2 kDa (NSP16) and 17.4 kDa (NSP10). Subunit NSP16 is fused with a polyhistidine tag at the N-terminus and subunit NSP10 is fused with a Twin Strep tag at the N-terminus.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4

Lagerung: -80 °C

Informationen zur Lagerung: The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.

Antigendetails

Target: SARS-CoV-2 NSP16, NSP10

Andere Bezeichnung: SARS-CoV-2 NSP16 & NSP10

Substanzklasse: Viral Protein

Hintergrund: NSP10, Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and 2'-O-methyltransferase (NSP16) activities. Therefore plays an essential role in viral mRNAs cap methylation. 2'-O-methyltransferase (NSP16) that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16, these interactions enhance nsp14 and nsp16 enzymatic activities.

Molekulargewicht: 35.3 kDa (NSP16) & 18.3 kDa (NSP10)