SARS-CoV-2 NSP3 Protein (His tag)

Produktnummer ABIN6952636

Produktdetails

Target: SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 NSP3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Verwendungszweck: SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag

Sequenz: AA 746-1060

Produktmerkmale: SARS-CoV-2 Papain-like Protease, His Tag is expressed from E.coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1). Predicted N-terminus: Met
This protein carries a polyhistidine tag at the N-terminus.

Reinheit: >95 % as determined by SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: This product is stable after storage at:
The product MUST be stored at -70°C or lower upon receipt,
-70°C for 3 months under sterile conditions.

Referenzen

Kim, Seiler, Jones, Ridout, Camp, Fabrizio, Jeevan, Miller, Throm, Ferrara, Fredrickson, Lowe, Wang, Odemuyiwa, Wan, Webby: "Antibody Responses to SARS-CoV-2 Antigens in Humans and Animals." in: Vaccines, Vol. 8, Issue 4, (2020) (PubMed).

Antigendetails

Target: SARS-CoV-2 NSP3 (NSP3) (SARS-CoV-2 Papain-Like Protease (NSP3))

Abstract: NSP3 Produkte

Synonyme: pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein

Substanzklasse: Viral Protein

Hintergrund: New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.

Molekulargewicht: 37.6 kDa

NCBI Accession: YP_009725299