SARS-CoV Spike Protein (RBD) (His tag)

Produktnummer ABIN6952627

Produktdetails

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Protein-Typ: Recombinant

Proteineigenschaft: RBD

Spezies: SARS Coronavirus (SARS-CoV)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV Spike Protein ist gelabelt mit His tag.

Applikation: ELISA, SDS-PAGE (SDS)

Verwendungszweck: SARS S protein RBD, His Tag (MALS verified)

Sequenz: AA 306-527

Produktmerkmale: SARS S protein RBD, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 306 - Phe 527 (Accession # AAP13567.1). Predicted N-terminus: Arg 306
This protein carries a polyhistidine tag at the C-terminus.

Reinheit: > 90 % as determined by SDS-PAGE.
> 95 % as determined by SEC-MALS.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Referenzen

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Antigendetails

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Andere Bezeichnung: SARS-Coronavirus Spike Protein (SARS-CoV S Produkte)

Substanzklasse: Viral Protein

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 26.9 kDa