SARS-CoV-2 Spike S1 Protein (His tag,AVI tag,Biotin)

Produktnummer ABIN6952457

Produktdetails

Target: SARS-CoV-2 Spike S1

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Sequenz: AA 16-685

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: Biotinylated 2019-nCoV (COVID-19) S1 protein, His,Avitag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). Predicted N-terminus: Val 16
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Reinheit: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: The protein has a calculated MW of 78.6 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.

Referenzen

Kaneko, Satta, Komuro, Muthukrishnan, Kakarla, Guo, An, Elahi, Kornblum, Liebeskind, Hsiai, Hinman: "Flow-Mediated Susceptibility and Molecular Response of Cerebral Endothelia to SARS-CoV-2 Infection." in: Stroke, Vol. 52, Issue 1, pp. 260-270, (2021) (PubMed).

Prieto-Fernández, Egia-Mendikute, Vila-Vecilla, Bosch, Barreira-Manrique, Lee, García-Del Río, Antoñana-Vildosola, Jiménez-Lasheras, Moreno-Cugnon, Jiménez-Barbero, Berra, Ereño-Orbea, Palazon: "Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate." in: Emerging microbes & infections, Vol. 10, Issue 1, pp. 1065-1076, (2021) (PubMed).

Ng, Attig, Bolland, Young, Major, Wrobel, Gamblin, Wack, Kassiotis: "Tissue-specific and interferon-inducible expression of nonfunctional ACE2 through endogenous retroelement co-option." in: Nature genetics, Vol. 52, Issue 12, pp. 1294-1302, (2020) (PubMed).

Antigendetails

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Produkte

Synonyme: E2 Protein, Surface Glycoprotein Protein, S Protein

Substanzklasse: Viral Protein

Hintergrund: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 78.6 kDa

Gen-ID: 43740568

UniProt: P0DTC2