SIGLEC15 Protein (AA 20-263) (His tag,AVI tag,Biotin)

Produktnummer ABIN6938941

Produktdetails

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 20-263

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SIGLEC15 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Sequenz: AA 20-263

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 25 mM MES,150 mM NaCl, pH 5.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Andere Bezeichnung: Siglec-15 (SIGLEC15 Produkte)

Synonyme: RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein

Hintergrund: Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

Molekulargewicht: 30.1 kDa

NCBI Accession: NP_998767