CD73 Protein (AA 27-549) (His tag,AVI tag,Biotin)

Produktnummer ABIN6810044

Produktdetails

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 27-549

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD73 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Sequenz: AA 27-549

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 120 mM NaCl, pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: CD73 (NT5E Produkte)

Synonyme: CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein

Hintergrund: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Molekulargewicht: 61.7 kDa

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process