PSME3 Protein (PSME3) (AA 1-254) (His tag)

Produktnummer ABIN7279625

Produktdetails

Target: PSME3

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-254

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PSME3 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: PSME3, 1-254aa, Human, His tag, E.coli

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl

Lagerung: 4 °C

Antigendetails

Target: PSME3

Andere Bezeichnung: PSME3 (PSME3 Produkte)

Synonyme: AA410043 Protein, AU020960 Protein, Ki Protein, PA28gamma Protein, REGgamma Protein, pa28g Protein, Ab2-371 Protein, PA28-gamma Protein, PA28G Protein, REG-GAMMA Protein, psme3b Protein, proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) Protein, proteasome activator subunit 3 Protein, proteasome activator subunit 3 S homeolog Protein, Psme3 Protein, PSME3 Protein, psme3.S Protein

Hintergrund: PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Proteasome activator complex subunit 3 isoform 1, Ki, PA28-gamma, PA28G, REG-GAMMA. NCBI no.: NP_005780

Molekulargewicht: 31.7kDa (274aa), confirmed by MALDI-TOF

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA